• Western blot analysis of extracts of various cell lines, using MMP9 antibody (STJ112990) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded human liver using MMP9 antibody (STJ112990) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse spleen using MMP9 antibody (STJ112990) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat spleen using MMP9 antibody (STJ112990) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MMP9 antibody (601-707) (STJ112990)

SKU:
STJ112990

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MMP9 (601-707) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MMP9
Gene ID: 4318
Uniprot ID: MMP9_HUMAN
Immunogen Region: 601-707
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 601-707 of human MMP9 (NP_004985.2).
Immunogen Sequence: LDKLGLGADVAQVTGALRSG RGKMLLFSGRRLWRFDVKAQ MVDPRSASEVDRMFPGVPLD THDVFQYREKAYFCQDRFYW RVSSRSELNQVDQVGYVTYD
Tissue Specificity Detected in neutrophils (at protein level). Produced by normal alveolar macrophages and granulocytes.
Post Translational Modifications Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. N- and O-glycosylated.
Function Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Protein Name Matrix Metalloproteinase-9
Mmp-9
92 Kda Gelatinase
92 Kda Type Iv Collagenase
Gelatinase B
Gelb Cleaved Into - 67 Kda Matrix Metalloproteinase-9 - 82 Kda Matrix Metalloproteinase-9
Database Links Reactome: R-HSA-1433557
Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Reactome: R-HSA-2022090
Reactome: R-HSA-3928665
Reactome: R-HSA-6785807
Reactome: R-HSA-6798695
Reactome: R-HSA-9009391
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Matrix Metalloproteinase-9 antibody
Anti-Mmp-9 antibody
Anti-92 Kda Gelatinase antibody
Anti-92 Kda Type Iv Collagenase antibody
Anti-Gelatinase B antibody
Anti-Gelb Cleaved Into - 67 Kda Matrix Metalloproteinase-9 - 82 Kda Matrix Metalloproteinase-9 antibody
Anti-MMP9 antibody
Anti-CLG4B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance