• Western blot analysis of lysates from Mouse spleen using MMP9 Rabbit polyclonal antibody (STJ11105299) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:90s.

Anti-MMP9 antibody (1-150) (STJ11105299)

SKU:
STJ11105299

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MMP9 (1-150) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Mmp9
Gene ID: 17395
Uniprot ID: MMP9_MOUSE
Immunogen Region: 1-150
Immunogen: Recombinant Protein corresponding to a sequence within amino acids 1-150 of mouse MMP9 (NP_038627.1).
Immunogen Sequence: RFQTFKGLKWDHHNITYWIQ NYSEDLPRDMIDDAFARAFA VWGEVAPLTFTRVYGPEADI VIQFGVAEHGDGYPFDGKDG LLAHAFPPGAGVQGDAHFDD DELWSLGKGVVIPTYYGNSN GAPCHFPFTFEGRSYSACTT DGRNDGTPWCSTTADYDKDG KFGFCPSERLYTEHGNGEGK PCVFPFIFEGRSYSACTTKG RSDGYRWCATTANYDQDKLY GFCPTRVDATVVGGNSAGE
Post Translational Modifications N- and O-glycosylated.
Function Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Protein Name Matrix Metalloproteinase-9
Mmp-9
92 Kda Gelatinase
92 Kda Type Iv Collagenase
Gelatinase B
Gelb
Database Links Reactome: R-MMU-1433557
Reactome: -MMU-1442490
Reactome: -MMU-1474228
Reactome: -MMU-1592389
Reactome: -MMU-2022090
Reactome: -MMU-3928665
Reactome: -MMU-6798695
Reactome: -MMU-9009391
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Matrix Metalloproteinase-9 antibody
Anti-Mmp-9 antibody
Anti-92 Kda Gelatinase antibody
Anti-92 Kda Type Iv Collagenase antibody
Anti-Gelatinase B antibody
Anti-Gelb antibody
Anti-Mmp9 antibody
Anti-Clg4b antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance