• Western blot analysis of lysates from LO2 cells, using MFF Rabbit polyclonal antibody (STJ114269) at 1:3000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-MFF antibody (15-170) (STJ114269)

SKU:
STJ114269

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MFF (15-170) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MFF
Gene ID: 56947
Uniprot ID: MFF_HUMAN
Immunogen Region: 15-170
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 15-170 of human MFF (NP_001263990.1).
Immunogen Sequence: SRAAFPSPTAAEMAEISRIQ YEMEYTEGISQRMRVPEKLK VAPPNADLEQGFQEGVPNAS VIMQVPERIVVAGNNEDVSF SRPADLDLIQSTPFKPLALK TPPRVLTLSERPLDFLDLER PPTTPQNEEIRAVGRLKRER SMSENAVRQNGQLVRN
Tissue Specificity Highly expressed in heart, kidney, liver, brain, muscle, and stomach.
Function Plays a role in mitochondrial and peroxisomal fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles.
Protein Name Mitochondrial Fission Factor
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Type Iv Membrane Protein
Peroxisome
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle
Alternative Antibody Names Anti-Mitochondrial Fission Factor antibody
Anti-MFF antibody
Anti-C2orf33 antibody
Anti-AD030 antibody
Anti-AD033 antibody
Anti-GL004 antibody

Information sourced from Uniprot.org

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