| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-METTL1 (1-276) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
METTL1 |
| Gene ID: |
4234 |
| Uniprot ID: |
TRMB_HUMAN |
| Immunogen Region: |
1-276 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-276 of human METTL1 (NP_005362.3). |
| Immunogen Sequence: |
MAAETRNVAGAEAPPPQKRY YRQRAHSNPMADHTLRYPVK PEEMDWSELYPEFFAPLTQN QSHDDPKDKKEKRAQAQVEF ADIGCGYGGLLVELSPLFPD TLILGLEIRVKVSDYVQDRI RALRAAPAGGFQNIACLRSN AMKHLPNFFYKGQLTKMFFL FPDPHFKRTKHKWRIISPTL LAEYAYVLRVGGLVYTITDV LELHDWMCTHFEEHPLFERV PLEDLSEDPVVGHLGTSTE |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylation at Ser-27 by PKB/AKT1 inactivates its methyltransferase activity via a steric interference mechanism in the active site that locally disrupts the catalytic center. Phosphorylation at Ser-27 does not affect the interaction with WDR4. |
| Function | Catalytic component of METTL1-WDR4 methyltransferase complex that mediates the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop. M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay. Also acts as a methyltransferase for a subset of internal N(7)-methylguanine in mRNAs. Internal N(7)-methylguanine methylation of mRNAs in response to stress promotes their relocalization to stress granules, thereby suppressing their translation. Also methylates a specific subset of miRNAs, such as let-7. N(7)-methylguanine methylation of let-7 miRNA promotes let-7 miRNA processing by disrupting an inhibitory secondary structure within the primary miRNA transcript (pri-miRNA). Acts as a regulator of embryonic stem cell self-renewal and differentiation. |
| Protein Name | TrnaGuanine-N(7--MethyltransferaseMethyltransferase-Like Protein 1MrnaGuanine-N(7--MethyltransferaseMirnaGuanine-N(7--MethyltransferaseTrnaGuanine(46-N(7-MethyltransferaseTrna(M7g46-Methyltransferase |
| Database Links | Reactome: R-HSA-6782315 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Trna antibodyAnti-Guanine-N(7--Methyltransferase antibodyAnti-Methyltransferase-Like Protein 1 antibodyAnti-Mrna antibodyAnti-Guanine-N(7--Methyltransferase antibodyAnti-Mirna antibodyAnti-Guanine-N(7--Methyltransferase antibodyAnti-Trna antibodyAnti-Guanine(46-N(7-Methyltransferase antibodyAnti-Trna(M7g46-Methyltransferase antibodyAnti-METTL1 antibodyAnti-C12orf1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
