• Immunohistochemistry analysis of MECP2 in paraffin-embedded mouse brain using [KO Validated] MECP2 Rabbit polyclonal antibody (STJ114895) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MECP2 in paraffin-embedded human gastric cancer using [KO Validated] MECP2 Rabbit polyclonal antibody (STJ114895) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and MECP2 knockout (KO) 293T cells, using [KO Validated] MECP2 Rabbit polyclonal antibody (STJ114895) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates using [KO Validated] MECP2 Rabbit polyclonal antibody (STJ114895) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MECP2 antibody (1-280) (STJ114895)

SKU:
STJ114895

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MECP2 (1-280) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MECP2
Gene ID: 4204
Uniprot ID: MECP2_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MECP2 (NP_004983.1).
Immunogen Sequence: MVAGMLGLREEKSEDQDLQG LKDKPLKFKKVKKDKKEEKE GKHEPVQPSAHHSAEPAEAG KAETSEGSGSAPAVPEASAS PKQRRSIIRDRGPMYDDPTL PEGWTRKLKQRKSGRSAGKY DVYLINPQGKAFRSKVELIA YFEKVGDTSLDPNDFDFTVT GRGSPSRREQKPPKKPKSPK APGTGRGRGRPKGSGTTRPK AATSEGVQVKRVLEKSPGKL LVKMPFQTSPGGKAEGGGA
Tissue Specificity Present in all adult somatic tissues tested.
Post Translational Modifications Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.
Function Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
Protein Name Methyl-Cpg-Binding Protein 2
Mecp-2 Protein
Mecp2
Database Links Reactome: R-HSA-8986944
Reactome: R-HSA-9022534
Reactome: R-HSA-9022535
Reactome: R-HSA-9022537
Reactome: R-HSA-9022538
Reactome: R-HSA-9022692
Reactome: R-HSA-9022699
Reactome: R-HSA-9022702
Reactome: R-HSA-9022707
Reactome: R-HSA-9022927
Reactome: R-HSA-9725371
Cellular Localisation Nucleus
Colocalized With Methyl-Cpg In The Genome
Colocalized With Tbl1x To The Heterochromatin Foci
Alternative Antibody Names Anti-Methyl-Cpg-Binding Protein 2 antibody
Anti-Mecp-2 Protein antibody
Anti-Mecp2 antibody
Anti-MECP2 antibody

Information sourced from Uniprot.org

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