| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MCM8 (591-840) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MCM8 |
| Gene ID: |
84515 |
| Uniprot ID: |
MCM8_HUMAN |
| Immunogen Region: |
591-840 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 591-840 of human MCM8 (NP_115874.3). |
| Immunogen Sequence: |
FILLDTPNEHHDHLLSEHVI AIRAGKQRTISSATVARMNS QDSNTSVLEVVSEKPLSERL KVVPGETIDPIPHQLLRKYI GYARQYVYPRLSTEAARVLQ DFYLELRKQSQRLNSSPITT RQLESLIRLTEARARLELRE EATKEDAEDIVEIMKYSMLG TYSDEFGNLDFERSQHGSGM SNRSTAKRFISALNNVAERT YNNIFQFHQLRQIAKELNIQ VADFENFIGSLNDQGYLLK |
| Tissue Specificity | Highest levels in placenta, lung and pancreas. Low levels in skeletal muscle and kidney. Expressed in various tumors with highest levels in colon and lung cancers. |
| Function | Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC). Probably by regulating HR, plays a key role during gametogenesis. Stabilizes MCM9 protein. |
| Protein Name | Dna Helicase Mcm8Minichromosome Maintenance 8 |
| Database Links | Reactome: R-HSA-113507Reactome: R-HSA-176187Reactome: R-HSA-176974Reactome: R-HSA-68689Reactome: R-HSA-68949Reactome: R-HSA-68962 |
| Cellular Localisation | NucleusChromosomeLocalizes To Nuclear FociLocalizes To Double-Stranded Dna BreaksBinds Chromatin Throughout The Cell Cycle |
| Alternative Antibody Names | Anti-Dna Helicase Mcm8 antibodyAnti-Minichromosome Maintenance 8 antibodyAnti-MCM8 antibodyAnti-C20orf154 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
