• Immunofluorescence analysis of paraffin-embedded rat brain using Myelin Basic Protein/MBP Rabbit polyclonal antibody (STJ11105502) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform microwave antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of paraffin-embedded mouse brain using Myelin Basic Protein/MBP Rabbit polyclonal antibody (STJ11105502) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform microwave antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of Myelin Basic Protein/MBP in paraffin-embedded Human brain tissue using Myelin Basic Protein/MBP Rabbit polyclonal antibody (STJ11105502) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from Mouse brain using Myelin Basic Protein/MBP Rabbit polyclonal antibody (STJ11105502) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time:0. 5s.

Anti-MBP antibody (1-304) (STJ11105502)

SKU:
STJ11105502

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Myelin Basic Protein (1-304) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MBP
Gene ID: 4155
Uniprot ID: MBP_HUMAN
Immunogen Region: 1-304
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-304 of human Myelin Basic Protein/MBP (NP_001020272.1).
Immunogen Sequence: MGNHAGKRELNAEKASTNSE TNRGESEKKRNLGELSRTTS EDNEVFGEADANQNNGTSSQ DTAVTDSKRTADPKNAWQDA HPADPGSRPHLIRLFSRDAP GREDNTFKDRPSESDELQTI QEDSAATSESLDVMASQKRP SQRHGSKYLATASTMDHARH GFLPRHRDTGILDSIGRFFG GDRGAPKRGSGKDSHHPART AHYGSLPQKSHGRTQDENPV VHFFKNIVTPRTPPPSQGK
Tissue Specificity MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
Post Translational Modifications Several charge isomers of MBP.C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form).are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic. The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. Proteolytically cleaved in B cell lysosomes by cathepsin CTSG which degrades the major immunogenic MBP epitope and prevents the activation of MBP-specific autoreactive T cells. Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1.
Function The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
Protein Name Myelin Basic Protein
Mbp
Myelin A1 Protein
Myelin Membrane Encephalitogenic Protein
Database Links Reactome: R-HSA-9619665
Cellular Localisation Myelin Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasmic Side Of Myelin
Isoform 3: Nucleus
Targeted To Nucleus In Oligodendrocytes
Alternative Antibody Names Anti-Myelin Basic Protein antibody
Anti-Mbp antibody
Anti-Myelin A1 Protein antibody
Anti-Myelin Membrane Encephalitogenic Protein antibody
Anti-MBP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance