• Western blot of rat cortical lysate showing specific immunolabeling of the ~18 kDa and ~22 kDa MBP protein.
  • Immunofluorescence of a section of rat cerebellum showing specific labeling of myelin basic protein (STJA0003695, 1:5000, green) in the oligodendrocytes and myelin sheathes around axons. The section is colabeled with Anti-NFL (red) labeling dendrites and axons of neuronal cells. The blue stains nuclear DNA.
  • Immunofluorescence of a perpendicular section from mouse spinal cord gray matter (upper left corner) and white matter (lower right corner) labeling myelin basic protein (:STJA0003695, magenta, 1:500) from oligodendrocytes and myelin sheaths. The blue is DAPI counterstaining nuclear DNA. Image kindly provided by Dr. Rodolfo Gatto and Dr. Gerardo Morfini, Department of Anatomy and Cell biology, University of Illinois at Chicago.
  • Immunofluorescence of a cross-section from mouse corpus callosum labeling myelin basic protein (: STJA0003695, magenta, 1:500) in oligodendrocytes and myelin sheaths. The blue is DAPI counterstaining nuclear DNA. Image kindly provided by Dr. Rodolfo Gatto and Dr. Gerardo Morfini, Department of Anatomy and Cell biology, University of Illinois at Chicago.
  • Immunostaining of cultured neonatal rat neurons and glia stained with anti-myelin basic protein antibody (STJA0003695, red, 1:500). The blue stain is to identify nuclear DNA. Note the antibody stains the myelin basic protein located in oligodendrocyte and myelin sheathes around axons in the cells.

Anti-MBP antibody (STJA0003695)

SKU:
STJA0003695-100

£394.50
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Host: Chicken
Applications: WB/IHC/ICC
Reactivity: Bovine/Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Chicken polyclonal antibody anti-MBP is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgY
Formulation: Total IgY fraction in PBS + 10 mM Sodium Azide.
Purification: This antibody was total igy fraction.
Dilution Range: WB 1:5000
IHC 1:500-1:5000
ICC 1:500-1:2000
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Mbp
Gene ID: 24547
Uniprot ID: MBP_RAT
Immunogen: Three peptide sequences conserved in higher vertebrate MBP proteins.
Tissue Specificity Found in both the central and the peripheral nervous system.
Post Translational Modifications As in other animals, several charge isomers may be produced as a result of optional post-translational modifications, such as phosphorylation of serine or threonine residues, deamidation of glutamine or asparagine residues, citrullination and methylation of arginine residues. Arg-131 was found to be 44% monomethylated and 11% symmetrically dimethylated. Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1. Proteolytically cleaved in B cell lysosomes by cathepsin CTSG which degrades the major immunogenic MBP epitope and prevents the activation of MBP-specific autoreactive T cells.
Function Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane.
Protein Name Myelin Basic Protein
Mbp
Cellular Localisation Myelin Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasmic Side Of Myelin
Alternative Antibody Names Anti-Myelin Basic Protein antibody
Anti-Mbp antibody
Anti-Mbp antibody

Information sourced from Uniprot.org

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