Anti-MBP antibody (STJA0003695)

SPECIFICATIONS
ClonalityPolyclonal
HostChicken
ConjugationUnconjugated
IsotypeIgY
ImmunogenThree peptide sequences conserved in higher vertebrate MBP proteins.
STJA0003695-100
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General Information

Short DescriptionChicken polyclonal anti-MBP for use in WB, IHC and ICC in Bovine, Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC/ICC
HostChicken
ReactivityBovine/Human/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgY
ConjugationUnconjugated
PurificationThis antibody was total igy fraction.
Dilution RangeWB 1:5000
IHC 1:500-1:5000
ICC 1:500-1:2000
FormulationTotal IgY fraction in PBS + 10 mM Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMbp
Gene ID24547
Uniprot IDMBP_RAT
ImmunogenThree peptide sequences conserved in higher vertebrate MBP proteins.

Additional Info

Tissue Specificity Found in both the central and the peripheral nervous system.
Post Translational Modifications As in other animals, several charge isomers may be produced as a result of optional post-translational modifications, such as phosphorylation of serine or threonine residues, deamidation of glutamine or asparagine residues, citrullination and methylation of arginine residues. Arg-131 was found to be 44% monomethylated and 11% symmetrically dimethylated. Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1. Proteolytically cleaved in B cell lysosomes by cathepsin CTSG which degrades the major immunogenic MBP epitope and prevents the activation of MBP-specific autoreactive T cells.
Function Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane.
Protein Name Myelin Basic Protein
Mbp
Database Links
Cellular Localisation Myelin Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasmic Side Of Myelin
Alternative Antibody Names Anti-Myelin Basic Protein antibody
Anti-Mbp antibody
Anti-Mbp antibody

Information sourced from Uniprot.org

Citations

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