• Western blot analysis of extracts of various cell lines, using MAP4K4 antibody (STJ113305) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MAP4K4 antibody (480-650) (STJ113305)

SKU:
STJ113305

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAP4K4 (480-650) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP4K4
Gene ID: 9448
Uniprot ID: M4K4_HUMAN
Immunogen Region: 480-650
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 480-650 of human MAP4K4 (NP_663719.2).
Immunogen Sequence: EVLQQQLLQEQAMLLHDHRR PHPQHSQQPPPPQQERSKPS FHAPEPKAHYEPADRAREVE DRFRKTNHSSPEAQSKQTGR VLEPPVPSRSESFSNGNSES VHPALQRPAEPQVPVRTTSR SPVLSRRDSPLQGSGQQNSQ AGQRNSTSSIEPRLLWERVE KLVPRPGSGSS
Tissue Specificity Widely expressed. Isoform 5 is abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
Function Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.
Protein Name Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4
Hpk/Gck-Like Kinase Hgk
Mapk/Erk Kinase Kinase Kinase 4
Mek Kinase Kinase 4
Mekkk 4
Nck-Interacting Kinase
Database Links Reactome: R-HSA-2559580
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 antibody
Anti-Hpk/Gck-Like Kinase Hgk antibody
Anti-Mapk/Erk Kinase Kinase Kinase 4 antibody
Anti-Mek Kinase Kinase 4 antibody
Anti-Mekkk 4 antibody
Anti-Nck-Interacting Kinase antibody
Anti-MAP4K4 antibody
Anti-HGK antibody
Anti-KIAA0687 antibody
Anti-NIK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance