• Western blot analysis of various lysates using MANEA Rabbit polyclonal antibody (STJ112654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MANEA antibody (30-100) (STJ112654)

SKU:
STJ112654

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MANEA (30-100) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MANEA
Gene ID: 79694
Uniprot ID: MANEA_HUMAN
Immunogen Region: 30-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-100 of human MANEA (NP_078917.2).
Immunogen Sequence: RPNTATFGAPFGLDLLPELH QRTIHLGKNFDFQKSDRINS ETNTKNLKSVEITMKPSKAS ELNLDELPPLN
Tissue Specificity Highly expressed in the liver and kidney. Expressed at lower levels in muscle, pancreas, heart, placenta, lung and brain.
Post Translational Modifications Undergoes proteolytic cleavage in the C-terminal region.
Protein Name Glycoprotein Endo-Alpha-1 -2-Mannosidase
Endo-Alpha Mannosidase
Endomannosidase
Hendo
Mandaselin
Database Links Reactome: R-HSA-964739
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Glycoprotein Endo-Alpha-1 -2-Mannosidase antibody
Anti-Endo-Alpha Mannosidase antibody
Anti-Endomannosidase antibody
Anti-Hendo antibody
Anti-Mandaselin antibody
Anti-MANEA antibody

Information sourced from Uniprot.org

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