| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Lrwd1 (250-350) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
LRWD1 |
| Gene ID: |
222229 |
| Uniprot ID: |
LRWD1_HUMAN |
| Immunogen Region: |
250-350 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Lrwd1 (NP_690852.1). |
| Immunogen Sequence: |
ASPSAQVEGSPVAGSDGSQP AVKLEPLHFLQCHSKNNSPQ DLETQLWACAFEPAWEEGAT SQTVATCGGEAVCVIDCQTG IVLHKYKAPGEEFFSVAWTA L |
| Tissue Specificity | Testis-specific. Drastically down-regulated in testis from patients with Sertoli cell-only syndrome (SCOS). |
| Post Translational Modifications | Ubiquitinated.undergoes 'Lys-48'-linked polyubiquitination leading to proteasomal degradation. Ubiquitination occurs within the WD repeats at the end of the G1 phase. Ubiquitination may be catalyzed by the CUL4-DDB1 E3 ubiquitin-protein ligase complex and other E3 ligases. |
| Function | Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability. |
| Protein Name | Leucine-Rich Repeat And Wd Repeat-Containing Protein 1Centromere Protein 33Cenp-33Origin Recognition Complex-Associated ProteinOrc-Associated ProteinOrca |
| Cellular Localisation | NucleusChromosomeCentromereTelomereCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeKinetochoreLocalizes To Heterochromatin During G1 PhaseRestricted To Centromeres Or Telomeres As Cells Progress Though S PhaseWhen Cells Enter MitosisRelocalizes To CentromeresRecruitment To Pericentric Heterochromatin Largely Depends On The Presence Of H3k9me3 |
| Alternative Antibody Names | Anti-Leucine-Rich Repeat And Wd Repeat-Containing Protein 1 antibodyAnti-Centromere Protein 33 antibodyAnti-Cenp-33 antibodyAnti-Origin Recognition Complex-Associated Protein antibodyAnti-Orc-Associated Protein antibodyAnti-Orca antibodyAnti-LRWD1 antibodyAnti-CENP-33 antibodyAnti-ORCA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
