| Host: |
NZ White Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse/Rat/Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Nz White Rabbit polyclonal antibody anti-LIMK2 (Internal) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Shipped as lyophilised. Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material. |
| Purification: |
Ammonium sulphate precipitation |
| Dilution Range: |
A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
LIMK2 |
| Gene ID: |
3985 |
| Uniprot ID: |
LIMK2_HUMAN |
| Immunogen Region: |
Internal |
| Specificity: |
Specific for LIMK2. |
| Immunogen: |
A synthetic peptide from the internal part of human LIMK2 (LIM domain kinase 2, LIMK-2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, mouse. |
| Function | Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP. Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. Suppresses ciliogenesis via multiple pathways.phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1. |
| Protein Name | Lim Domain Kinase 2Limk-2 |
| Database Links | Reactome: R-HSA-3928662Reactome: R-HSA-416572Reactome: R-HSA-5627117 |
| Cellular Localisation | CytoplasmCytoskeletonSpindleMicrotubule Organizing CenterCentrosomeIsoform Limk2a: CytoplasmNucleusIsoform Limk2b: CytoplasmPerinuclear RegionMainly Present In The Cytoplasm And Is Scarcely Translocated To The Nucleus |
| Alternative Antibody Names | Anti-Lim Domain Kinase 2 antibodyAnti-Limk-2 antibodyAnti-LIMK2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
