• Immunofluorescence analysis of L929 cells using LIMK2 Rabbit polyclonal antibody (STJ27362) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using LIMK2 antibody (STJ27362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-LIMK2 antibody (1-240) (STJ27362)

SKU:
STJ27362

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LIMK2 (1-240) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LIMK2
Gene ID: 3985
Uniprot ID: LIMK2_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human LIMK2 (NP_001026971.1).
Immunogen Sequence: MGSYLSVPAYFTSRDLFRCS ECQDSLTNWYYEKDGKLYCP KDYWGKFGEFCHGCSLLMTG PFMVAGEFKYHPECFACMSC KVIIEDGDAYALVQHATLYC GKCHNEVVLAPMFERLSTES VQEQLPYSVTLISMPATTEG RRGFSVSVESACSNYATTVQ VKEVNRMHISPNNRNAIHPG DRILEINGTPVRTLRVEEVE DAISQTSQTLQLLIEHDPVS QRLDQLRLEARLAPHMQNA
Post Translational Modifications Phosphorylated on serine and/or threonine residues by ROCK1.
Function Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP. Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. Suppresses ciliogenesis via multiple pathways.phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1.
Protein Name Lim Domain Kinase 2
Limk-2
Database Links Reactome: R-HSA-3928662
Reactome: R-HSA-416572
Reactome: R-HSA-5627117
Cellular Localisation Cytoplasm
Cytoskeleton
Spindle
Microtubule Organizing Center
Centrosome
Isoform Limk2a: Cytoplasm
Nucleus
Isoform Limk2b: Cytoplasm
Perinuclear Region
Mainly Present In The Cytoplasm And Is Scarcely Translocated To The Nucleus
Alternative Antibody Names Anti-Lim Domain Kinase 2 antibody
Anti-Limk-2 antibody
Anti-LIMK2 antibody

Information sourced from Uniprot.org

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