• Immunofluorescence analysis of U2OS cells using LDHA Rabbit polyclonal antibody (STJ11104512) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF7 cells using LDHA Rabbit polyclonal antibody (STJ11104512) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using LDHA Rabbit polyclonal antibody (STJ11104512) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using LDHA Rabbit polyclonal antibody (STJ11104512) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using LDHA Rabbit polyclonal antibody (STJ11104512) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of HeLa, using LDHA Rabbit polyclonal antibody (STJ11104512) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-LDHA antibody (1-332) (STJ11104512)

SKU:
STJ11104512

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-L-Lactate Dehydrogenase A Chain (1-332) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LDHA
Gene ID: 3939
Uniprot ID: LDHA_HUMAN
Immunogen Region: 1-332
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-332 of human LDHA (NP_005557.1).
Immunogen Sequence: MATLKDQLIYNLLKEEQTPQ NKITVVGVGAVGMACAISIL MKDLADELALVDVIEDKLKG EMMDLQHGSLFLRTPKIVSG KDYNVTANSKLVIITAGARQ QEGESRLNLVQRNVNIFKFI IPNVVKYSPNCKLLIVSNPV DILTYVAWKISGFPKNRVIG SGCNLDSARFRYLMGERLGV HPLSCHGWVLGEHGDSSVPV WSGMNVAGVSLKTLHPDLGT DKDKEQWKEVHKQVVESAY
Tissue Specificity Predominantly expressed in anaerobic tissues such as skeletal muscle and liver.
Post Translational Modifications ISGylated.
Function Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Protein Name L-Lactate Dehydrogenase A Chain
Ldh-A
Cell Proliferation-Inducing Gene 19 Protein
Ldh Muscle Subunit
Ldh-M
Renal Carcinoma Antigen Ny-Ren-59
Database Links Reactome: R-HSA-70268
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-L-Lactate Dehydrogenase A Chain antibody
Anti-Ldh-A antibody
Anti-Cell Proliferation-Inducing Gene 19 Protein antibody
Anti-Ldh Muscle Subunit antibody
Anti-Ldh-M antibody
Anti-Renal Carcinoma Antigen Ny-Ren-59 antibody
Anti-LDHA antibody
Anti-PIG19 antibody

Information sourced from Uniprot.org

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