• Western blot analysis of extracts of various cell lines, using LCN1 antibody (STJ111870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human prostate using LCN1 antibody (STJ111870) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human prostate using LCN1 antibody (STJ111870) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using LCN1 antibody (STJ111870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-LCN1 antibody (19-176) (STJ111870)

SKU:
STJ111870

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LCN1 (19-176) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LCN1
Gene ID: 3933
Uniprot ID: LCN1_HUMAN
Immunogen Region: 19-176
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-176 of human LCN1 (NP_002288.1).
Immunogen Sequence: HHLLASDEEIQDVSGTWYLK AMTVDREFPEMNLESVTPMT LTTLEGGNLEAKVTMLISGR CQEVKAVLEKTDEPGKYTAD GGKHVAYIIRSHVKDHYIFY CEGELHGKPVRGVKLVGRDP KNNLEALEDFEKAAGARGLS TESILIPRQSETCSPGSD
Tissue Specificity Mainly expressed in lachrymal and salivary glands. Also expressed in the prostate.
Function Could play a role in taste reception. Could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. Exhibits an extremely wide ligand pocket.
Protein Name Lipocalin-1
Tear Lipocalin
Tlc
Tear Prealbumin
Tp
Von Ebner Gland Protein
Veg Protein
Database Links Reactome: R-HSA-804914
Cellular Localisation Secreted
Alternative Antibody Names Anti-Lipocalin-1 antibody
Anti-Tear Lipocalin antibody
Anti-Tlc antibody
Anti-Tear Prealbumin antibody
Anti-Tp antibody
Anti-Von Ebner Gland Protein antibody
Anti-Veg Protein antibody
Anti-LCN1 antibody
Anti-VEGP antibody

Information sourced from Uniprot.org

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