• Immunohistochemistry analysis of L3MBTL3 in paraffin-embedded human esophageal cancer using L3MBTL3 Rabbit polyclonal antibody (STJ29428) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of L3MBTL3 in paraffin-embedded human breast cancer using L3MBTL3 Rabbit polyclonal antibody (STJ29428) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse lung, using L3MBTL3 Rabbit polyclonal antibody (STJ29428) at 1:4000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-L3MBTL3 antibody (1-255) (STJ29428)

SKU:
STJ29428

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:2000-1:4000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: L3MBTL3
Gene ID: 84456
Uniprot ID: LMBL3_HUMAN
Immunogen Region: 1-255
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human L3MBTL3 (NP_115814.1).
Immunogen Sequence: MTESASSTSGQEFDVFSVMD WKDGVGTLPGSDLKFRVNEF GALEVITDENEMENVKKATA TTTWMVPTAQEAPTSPPSSR PVFPPAYWTSPPGCPTVFSE KTGMPFRLKDPVKVEGLQFC ENCCQYGNVDECLSGGNYCS QNCARHIKDKDQKEERDVEE DNEEEDPKCSRKKKPKLSLK ADTKEDGEERDDEMENKQDV RILRGSQRARRKRRGDSAVL KQGLPPKGKKAWCWASYLE
Function Is a negative regulator of Notch target genes expression, required for RBPJ-mediated transcriptional repression. It recruits KDM1A to Notch-responsive elements and promotes KDM1A-mediated H3K4me demethylation. Involved in the regulation of ubiquitin-dependent degradation of a set of methylated non-histone proteins, including SOX2, DNMT1 and E2F1. It acts as an adapter recruiting the CRL4-DCAF5 E3 ubiquitin ligase complex to methylated target proteins. Required for normal maturation of myeloid progenitor cells.
Protein Name Lethal(3malignant Brain Tumor-Like Protein 3
H-L(3mbt-Like Protein 3
L(3mbt-Like Protein 3
L3mbt-Like 3
Mbt-1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Lethal(3malignant Brain Tumor-Like Protein 3 antibody
Anti-H-L(3mbt-Like Protein 3 antibody
Anti-L(3mbt-Like Protein 3 antibody
Anti-L3mbt-Like 3 antibody
Anti-Mbt-1 antibody
Anti-L3MBTL3 antibody
Anti-KIAA1798 antibody
Anti-MBT1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance