• 1) Input: Hela Cell Lysate , 2) IP product: IP dilute 1:200
  • Immunofluorescence analysis of Human-breast tissue. 1, CK17 monoclonal antibody (10A1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Western blot analysis of 1) Hela, 2) MCF7, 3) 293T, diluted at 1:2000.
  • Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, CK17 monoclonal antibody (10A1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-KRT17 antibody [10A1] (STJ96983)

SKU:
STJ96983

€57.50 - €282.50
Current Stock:
Host: Mouse
Applications: WB/IHC/IF/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Keratin, type I cytoskeletal 17 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: 10A1
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 1:1000
IP 1:200
IF 1:200
IHC 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT17
Gene ID: 3872
Uniprot ID: K1C17_HUMAN
Specificity: The antibody detects CK17 endogenous proteins.
Immunogen: Synthetic Peptide of CK17
Post Translational Modifications Phosphorylation at Ser-44 occurs in a growth- and stress-dependent fashion in skin keratinocytes, it has no effect on filament organization.
Function Type I keratin involved in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial 'stem cells'. Acts as a promoter of epithelial proliferation by acting a regulator of immune response in skin: promotes Th1/Th17-dominated immune environment contributing to the development of basaloid skin tumors. May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation.
Protein Name Keratin - Type I Cytoskeletal 17
39.1
Cytokeratin-17
Ck-17
Keratin-17
K17
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 17 antibody
Anti-39.1 antibody
Anti-Cytokeratin-17 antibody
Anti-Ck-17 antibody
Anti-Keratin-17 antibody
Anti-K17 antibody
Anti-KRT17 antibody

Information sourced from Uniprot.org

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