• Immunohistochemistry analysis of paraffin-embedded mouse lung using KLK11 antibody (STJ114439) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human prostate using KLK11 antibody (STJ114439) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using KLK11 antibody (STJ114439) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of 22Rv1 cells, using KLK11 antibody (STJ114439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 20s.

Anti-KLK11 antibody (1-250) (STJ114439)

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STJ114439

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KLK11 (1-250) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KLK11
Gene ID: 11012
Uniprot ID: KLK11_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human KLK11 (NP_001129504.1).
Immunogen Sequence: MRILQLILLALATGLVGGET RIIKGFECKPHSQPWQAALF EKTRLLCGATLIAPRWLLTA AHCLKPRYIVHLGQHNLQKE EGCEQTRTATESFPHPGFNN SLPNKDHRNDIMLVKMASPV SITWAVRPLTLSSRCVTAGT SCLISGWGSTSSPQLRLPHT LRCANITIIEHQKCENAYPG NITDTMVCASVQEGGKDSCQ GDSGGPLVCNQSLQGIISWG QDPCAITRKPGVYTKVCKY
Tissue Specificity Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level).
Post Translational Modifications About 40% of KLK11 is inactivated by internal cleavage after Arg-188. This proteolytic inactivation may be effected by plasminogen.
Function Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues.
Protein Name Kallikrein-11
Hk11
Hippostasin
Serine Protease 20
Trypsin-Like Protease Cleaved Into - Kallikrein-11 Inactive Chain 1 - Kallikrein-11 Inactive Chain 2
Cellular Localisation Isoform 1: Secreted
Isoform 2: Golgi Apparatus
Alternative Antibody Names Anti-Kallikrein-11 antibody
Anti-Hk11 antibody
Anti-Hippostasin antibody
Anti-Serine Protease 20 antibody
Anti-Trypsin-Like Protease Cleaved Into - Kallikrein-11 Inactive Chain 1 - Kallikrein-11 Inactive Chain 2 antibody
Anti-KLK11 antibody
Anti-PRSS20 antibody
Anti-TLSP antibody
Anti-UNQ649 antibody
Anti-PRO1279 antibody

Information sourced from Uniprot.org

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