| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-KLF4 (180-479) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KLF4 |
| Gene ID: |
9314 |
| Uniprot ID: |
KLF4_HUMAN |
| Immunogen Region: |
180-479 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 180-479 of human KLF4 (NP_004226.3). |
| Immunogen Sequence: |
PPTAPFNLADINDVSPSGGF VAELLRPELDPVYIPPQQPQ PPGGGLMGKFVLKASLSAPG SEYGSPSVISVSKGSPDGSH PVVVAPYNGGPPRTCPKIKQ EAVSSCTHLGAGPPLSNGHR PAAHDFPLGRQLPSRTTPTL GLEEVLSSRDCHPALPLPPG FHPHPGPNYPSFLPDQMQPQ VPPLHYQELMPPGSCMPEEP KPKRGRRSWPRKRTATHTCD YAGCGKTYTKSSHLKAHLR |
| Post Translational Modifications | Ubiquitinated. 'Lys-48'-linked ubiquitinated and targeted for proteasomal degradation by the SCF(BTRC) E3 ubiquitin-protein ligase complex, thereby negatively regulating cell pluripotency maintenance and embryogenesis. Polyglutamylated by TTLL1 and TTLL4 at Glu-411, which inhibits KLF4 binding with E3 ligase component BTRC, thereby impeding ubiquitination. Deglutamylated by CCP1 and CCP6.deglutamylation promotes KLF4 ubiquitination. KLF4 glutamylation state plays a critical role in the regulation of its function in cell reprogramming, pluripotency maintenance and embryogenesis. |
| Function | Transcription factor.can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. |
| Protein Name | Krueppel-Like Factor 4Epithelial Zinc Finger Protein EzfGut-Enriched Krueppel-Like Factor |
| Database Links | Reactome: R-HSA-381340Reactome: R-HSA-422085Reactome: R-HSA-452723Reactome: R-HSA-9617828 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Krueppel-Like Factor 4 antibodyAnti-Epithelial Zinc Finger Protein Ezf antibodyAnti-Gut-Enriched Krueppel-Like Factor antibodyAnti-KLF4 antibodyAnti-EZF antibodyAnti-GKLF antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
