• Western blot analysis of extracts of A-549 cells, using Integrin alpha 5 (ITGA5/CD49e) antibody (STJ11101606) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using Integrin alpha 5 (ITGA5/CD49e) antibody (STJ11101606) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using Integrin alpha 5 (ITGA5/CD49e) antibody (STJ11101606) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using Integrin alpha 5 (ITGA5/CD49e) rabbit monoclonal antibody (STJ11101606) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of Rat colon using Integrin alpha 5 (ITGA5/CD49e) rabbit monoclonal antibody (STJ11101606) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Flow cytometry:1X10^6 Daudi cells (negative control, left) and K-562 cells (right) were intracellularly-stained with Integrin alpha 5 (ITGA5/CD49e) rabbit monoclonal antibody (STJ11101606, 5 Mu g/mL, green line) or rabbit IgG isotype control (5 Mu g/mL, blue line) , followed by Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
  • Flow cytometry:1X10^6 Daudi cells (negative control, left) and U-87MG cells (right) were intracellularly-stained with Integrin alpha 5 (ITGA5/CD49e) rabbit monoclonal antibody (STJ11101606, 5 Mu g/mL, green line) or rabbit IgG isotype control (5 Mu g/mL, blue line) , followed by Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).

Anti-ITGA5 antibody (950-1049) [S6MR] (STJ11101606)

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STJ11101606

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Integrin alpha 5 (950-1049) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
FC (Intra) 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITGA5
Gene ID: 3678
Uniprot ID: ITA5_HUMAN
Immunogen Region: 950-1049
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 950-1049 of human Integrin alpha 5 (ITGA5/CD49e) (P08648).
Immunogen Sequence: HQPFSLQCEAVYKALKMPYR ILPRQLPQKERQVATAVQWT KAEGSYGVPLWIIILAILFG LLLLGLLIYILYKLGFFKRS LPYGTAMEKAQLKPPATSDA
Tissue Specificity Expressed in placenta (at protein level).
Post Translational Modifications Proteolytic cleavage by PCSK5 mediates activation of the precursor.
Function Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5:ITGB1 acts as a receptor for fibronectin (FN1) and mediates R-G-D-dependent cell adhesion to FN1. ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling. ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling. (Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human metapneumovirus. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human parvovirus B19. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Protein Name Integrin Alpha-5
Cd49 Antigen-Like Family Member E
Fibronectin Receptor Subunit Alpha
Integrin Alpha-F
Vla-5
Cd Antigen Cd49e Cleaved Into - Integrin Alpha-5 Heavy Chain - Integrin Alpha-5 Light Chain
Database Links Reactome: R-HSA-1566948
Reactome: R-HSA-1566977
Reactome: R-HSA-202733
Reactome: R-HSA-216083
Reactome: R-HSA-445144
Reactome: R-HSA-8941332
Reactome: R-HSA-9634597
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Junction
Focal Adhesion
Alternative Antibody Names Anti-Integrin Alpha-5 antibody
Anti-Cd49 Antigen-Like Family Member E antibody
Anti-Fibronectin Receptor Subunit Alpha antibody
Anti-Integrin Alpha-F antibody
Anti-Vla-5 antibody
Anti-Cd Antigen Cd49e Cleaved Into - Integrin Alpha-5 Heavy Chain - Integrin Alpha-5 Light Chain antibody
Anti-ITGA5 antibody
Anti-FNRA antibody

Information sourced from Uniprot.org

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