• Western blot analysis of lysates from 293T cells, using IRF5 Rabbit polyclonal antibody (STJ118816) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-IRF5 antibody (1-200) (STJ118816)

SKU:
STJ118816

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IRF5 (1-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IRF5
Gene ID: 3663
Uniprot ID: IRF5_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human IRF5 (NP_001092097.2).
Immunogen Sequence: MNQSIPVAPTPPRRVRLKPW LVAQVNSCQYPGLQWVNGEK KLFCIPWRHATRHGPSQDGD NTIFKAWAKETGKYTEGVDE ADPAKWKANLRCALNKSRDF RLIYDGPRDMPPQPYKIYEV CSNGPAPTDSQPPEDYSFGA GEEEEEEEELQRMLPSLSLT EDVKWPPTLQPPTLRPPTLQ PPTLQPPVVLGPPAPDPSPL
Post Translational Modifications Phosphorylation of serine and threonine residues by IKBKB in a C-terminal autoinhibitory region, stimulates dimerization, transport into the nucleus, assembly with the coactivator CBP/EP300 and initiation of transcription. 'Lys-63'-linked polyubiquitination by TRAF6 is required for activation.
Function Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88-dependent pathway. Key transcription factor regulating the IFN response during SARS-CoV-2 infection.
Protein Name Interferon Regulatory Factor 5
Irf-5
Database Links Reactome: R-HSA-877300
Reactome: R-HSA-909733
Cellular Localisation Cytoplasm
Nucleus
Shuttles Between The Nucleus And The Cytoplasm: Upon Activation By The Tlr Adapter Myd88 And Subsequent Phosphorylation
Translocates To The Nucleus
Alternative Antibody Names Anti-Interferon Regulatory Factor 5 antibody
Anti-Irf-5 antibody
Anti-IRF5 antibody

Information sourced from Uniprot.org

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