• Western blot analysis of extracts of various cell lines, using ILF3 rabbit monoclonal antibody (STJ11102341) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of ILF3 in paraffin-embedded mouse brain tissue using ILF3 Rabbit monoclonal antibody (STJ11102341) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded rat testis using ILF3 rabbit monoclonal antibody (STJ11102341) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of ILF3 in paraffin-embedded human liver tissue using ILF3 Rabbit monoclonal antibody (STJ11102341) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded human placenta using ILF3 rabbit monoclonal antibody (STJ11102341) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of ILF3 in paraffin-embedded rat liver tissue using ILF3 Rabbit monoclonal antibody (STJ11102341) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded mouse kidney using ILF3 rabbit monoclonal antibody (STJ11102341) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of ILF3 in paraffin-embedded mouse colon tissue using ILF3 Rabbit monoclonal antibody (STJ11102341) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ILF3 in paraffin-embedded rat colon tissue using ILF3 Rabbit monoclonal antibody (STJ11102341) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ILF3 in paraffin-embedded mouse testis tissue using ILF3 Rabbit monoclonal antibody (STJ11102341) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ILF3 in paraffin-embedded mouse lung tissue using ILF3 Rabbit monoclonal antibody (STJ11102341) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ILF3 in paraffin-embedded human thyroid cancer tissue using ILF3 Rabbit monoclonal antibody (STJ11102341) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ILF3 in paraffin-embedded human cervix cancer tissue using ILF3 Rabbit monoclonal antibody (STJ11102341) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ILF3 in paraffin-embedded human colon tissue using ILF3 Rabbit monoclonal antibody (STJ11102341) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ILF3 in paraffin-embedded human colon carcinoma tissue using ILF3 Rabbit monoclonal antibody (STJ11102341) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using ILF3 Rabbit monoclonal antibody (STJ11102341) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ILF3 antibody (1-100) [S1MR] (STJ11102341)

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STJ11102341

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ILF3 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ILF3
Gene ID: 3609
Uniprot ID: ILF3_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ILF3 (Q12906).
Immunogen Sequence: MRPMRIFVNDDRHVMAKHSS VYPTQEELEAVQNMVSHTER ALKAVSDWIDEQEKGSSEQA ESDNMDVPPEDDSKEGAGEQ KTEHMTRTLRGVMRVGLVAK
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylated at Thr-188 and Thr-315 by PKR in response to certain RNA viruses. This phosphorylation results in the dissociation of ILF2 from the ILF2-ILF3 complex resulting in a cytoplasmic sequestration of ILF3 where it can bind to viral RNAs and impede viral replication. Methylated by protein arginine N-methyltransferase 1. Ubiquitinated at Lys-297 in a TRIM47-dependent manner.this 'Lys-48'-linked ubiquitination promotes ILF3 degradation.
Function RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs. As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response. Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs. (Microbial infection) Plays a positive role in HIV-1 virus production by binding to and thereby stabilizing HIV-1 RNA, together with ILF3.
Protein Name Interleukin Enhancer-Binding Factor 3
Double-Stranded Rna-Binding Protein 76
Drbp76
M-Phase Phosphoprotein 4
Mpp4
Nuclear Factor Associated With Dsrna
Nfar
Nuclear Factor Of Activated T-Cells 90 Kda
Nf-At-90
Translational Control Protein 80
Tcp80
Database Links Reactome: R-HSA-9762293
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
Localizes In The Cytoplasm In Response To Viral Infection
The Unphosphorylated Form Is Retained In The Nucleus By Ilf2
Phosphorylation At Thr-188 And Thr-315 Causes The Dissociation Of Ilf2 From The Ilf2-Ilf3 Complex Resulting In A Cytoplasmic Sequestration Of Ilf3
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Alternative Antibody Names Anti-Interleukin Enhancer-Binding Factor 3 antibody
Anti-Double-Stranded Rna-Binding Protein 76 antibody
Anti-Drbp76 antibody
Anti-M-Phase Phosphoprotein 4 antibody
Anti-Mpp4 antibody
Anti-Nuclear Factor Associated With Dsrna antibody
Anti-Nfar antibody
Anti-Nuclear Factor Of Activated T-Cells 90 Kda antibody
Anti-Nf-At-90 antibody
Anti-Translational Control Protein 80 antibody
Anti-Tcp80 antibody
Anti-ILF3 antibody
Anti-DRBF antibody
Anti-MPHOSPH4 antibody
Anti-NF90 antibody

Information sourced from Uniprot.org

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