| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-IL37 (1-218) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
IL37 |
| Gene ID: |
27178 |
| Uniprot ID: |
IL37_HUMAN |
| Immunogen Region: |
1-218 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human IL37 (NP_055254.2). |
| Immunogen Sequence: |
MSFVGENSGVKMGSEDWEKD EPQCCLEDPAGSPLEPGPSL PTMNFVHTSPKVKNLNPKKF SIHDQDHKVLVLDSGNLIAV PDKNYIRPEIFFALASSLSS ASAEKGSPILLGVSKGEFCL YCDKDKGQSHPSLQLKKEKL MKLAAQKESARRPFIFYRAQ VGSWNMLESAAHPGWFICTS CNCNEPVGVTDKFENRKHIE FSFQPVCKAEMSPSEVSD |
| Tissue Specificity | In general, low constitutive expression, if any, in healthy tissues.high expression in inflammatory counterparts, including in synovial tissues from individuals with active rheumatoid arthritis. Isoform A, isoform B and isoform C are expressed in testis, colon, placenta, lung and lymph node. Isoform D and isoform E were found only in testis and bone marrow. Whereas only isoform A is found in brain, only isoform B in kidney and only isoform C in heart. |
| Post Translational Modifications | Proteolytically converted to the mature form by CASP1. |
| Function | Immune regulatory cytokine that acts as a suppressor of innate inflammatory and immune responses involved in curbing excessive inflammation. Signaling can occur via two mechanisms, intracellularly through nuclear translocation with SMAD3 and extracellularly after secretion and binding to its receptor composed of IL18R1 and IL18RAP. Suppresses, or reduces, pro-inflammatory cytokine production, including IL1A and IL6, as well as CCL12, CSF1, CSF2, CXCL13, IL1B, IL23A and IL1RN, but spares anti-inflammatory cytokines. Inhibits dendritic cell activation. |
| Protein Name | Interleukin-37Il-37Fil1 ZetaIl-1xInterleukin-1 Family Member 7Il-1f7Interleukin-1 Homolog 4Il-1hIl-1h4Interleukin-1 ZetaIl-1 ZetaInterleukin-1-Related ProteinIl-1rp1 |
| Database Links | Reactome: R-HSA-9008059Reactome: R-HSA-9012546 |
| Cellular Localisation | CytoplasmCytosolNucleusSecretedStimulation With Il1b Leads To Colocalization With Smad3 Mostly In Perinuclear RegionsOnly The Casp1-Cleaved Mature Form Translocates Into The Nucleus Upon Lps StimulationThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion |
| Alternative Antibody Names | Anti-Interleukin-37 antibodyAnti-Il-37 antibodyAnti-Fil1 Zeta antibodyAnti-Il-1x antibodyAnti-Interleukin-1 Family Member 7 antibodyAnti-Il-1f7 antibodyAnti-Interleukin-1 Homolog 4 antibodyAnti-Il-1h antibodyAnti-Il-1h4 antibodyAnti-Interleukin-1 Zeta antibodyAnti-Il-1 Zeta antibodyAnti-Interleukin-1-Related Protein antibodyAnti-Il-1rp1 antibodyAnti-IL37 antibodyAnti-FIL1Z antibodyAnti-IL1F7 antibodyAnti-IL1H4 antibodyAnti-IL1RP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and HPRT1 knockout (KO) 293T cells, using [KO Validated] HPRT1 Rabbit polyclonal antibody (STJ27659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/101287/388854/STJ27659_1__09600.1713158416.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HPRT1 knockout (KO) 293T cells, using [KO Validated] HPRT1 Rabbit polyclonal antibody (STJ27659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
