• Western blot analysis of extracts of THP-1 cells, using IFNB1 antibody (STJ24132) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-IFNB1 antibody (30-187) (STJ24132)

SKU:
STJ24132

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IFNB1 (30-187) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IFNB1
Gene ID: 3456
Uniprot ID: IFNB_HUMAN
Immunogen Region: 30-187
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-187 of human IFN-beta (NP_002167.1).
Immunogen Sequence: LQRSSNFQCQKLLWQLNGRL EYCLKDRMNFDIPEEIKQLQ QFQKEDAALTIYEMLQNIFA IFRQDSSSTGWNETIVENLL ANVYHQINHLKTVLEEKLEK EDFTRGKLMSSLHLKRYYGR ILHYLKAKEYSHCAWTIVRV EILRNFYFINRLTGYLRN
Function Type I interferon cytokine that plays a key role in the innate immune response to infection, developing tumors and other inflammatory stimuli. Signals via binding to high-affinity (IFNAR2) and low-affinity (IFNAR1) heterodimeric receptor, activating the canonical Jak-STAT signaling pathway resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response, such as antiviral proteins, regulators of cell proliferation and differentiation, and immunoregulatory proteins. Signals mostly via binding to a IFNAR1-IFNAR2 heterodimeric receptor, but can also function with IFNAR1 alone and independently of Jak-STAT pathways. Elicits a wide variety of responses, including antiviral and antibacterial activities, and can regulate the development of B-cells, myelopoiesis and lipopolysaccharide (LPS)-inducible production of tumor necrosis factor. Plays a role in neuronal homeostasis by regulating dopamine turnover and protecting dopaminergic neurons: acts by promoting neuronal autophagy and alpha-synuclein clearance, thereby preventing dopaminergic neuron loss. IFNB1 is more potent than interferon-alpha (IFN-alpha) in inducing the apoptotic and antiproliferative pathways required for control of tumor cell growth.
Protein Name Interferon Beta
Ifn-Beta
Fibroblast Interferon
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-909733
Reactome: R-HSA-912694
Reactome: R-HSA-918233
Reactome: R-HSA-933541
Reactome: R-HSA-9705671
Reactome: R-HSA-983231
Cellular Localisation Secreted
Alternative Antibody Names Anti-Interferon Beta antibody
Anti-Ifn-Beta antibody
Anti-Fibroblast Interferon antibody
Anti-IFNB1 antibody
Anti-IFB antibody
Anti-IFNB antibody

Information sourced from Uniprot.org

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