• Western blot analysis of various lysates, using IDH1 rabbit monoclonal antibody (STJ11102254) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human kidney using IDH1 rabbit monoclonal antibody (STJ11102254) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human testis using IDH1 rabbit monoclonal antibody (STJ11102254) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using IDH1 rabbit monoclonal antibody (STJ11102254) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using IDH1 rabbit monoclonal antibody (STJ11102254) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Confocal imaging of NIH/3T3 cells using IDH1 rabbit monoclonal antibody (STJ11102254, dilution1:100) (Red). The cells were counterstained with Alpha-Tubulin (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x

Anti-IDH1 antibody (100-200) [S4MR] (STJ11102254)

SKU:
STJ11102254

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-IDH1 (100-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
IHC-P 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IDH1
Gene ID: 3417
Uniprot ID: IDHC_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human IDH1 (NP_005887.2).
Immunogen Sequence: RNILGGTVFREAIICKNIPR LVSGWVKPIIIGRHAYGDQY RATDFVVPGPGKVEITYTPS DGTQKVTYLVHNFEEGGGVA MGMYNQDKSIEDFAHSSFQM A
Post Translational Modifications Acetylation at Lys-374 dramatically reduces catalytic activity.
Function Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase. Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways. May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency.
Protein Name Isocitrate Dehydrogenase Nadp Cytoplasmic
Idh
Idh1
Cytosolic Nadp-Isocitrate Dehydrogenase
Idpc
Nadp(+-Specific Icdh
Oxalosuccinate Decarboxylase
Database Links Reactome: R-HSA-2978092
Reactome: R-HSA-389542
Reactome: R-HSA-6798695
Reactome: R-HSA-9033241
Reactome: R-HSA-9818025
Cellular Localisation Cytoplasm
Cytosol
Peroxisome
Alternative Antibody Names Anti-Isocitrate Dehydrogenase Nadp Cytoplasmic antibody
Anti-Idh antibody
Anti-Idh1 antibody
Anti-Cytosolic Nadp-Isocitrate Dehydrogenase antibody
Anti-Idpc antibody
Anti-Nadp(+-Specific Icdh antibody
Anti-Oxalosuccinate Decarboxylase antibody
Anti-IDH1 antibody
Anti-PICD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance