• Western blot analysis of extracts of various cell lines, using HSPB8/HSP22/HSP22 antibody (STJ24102) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded rat heart using HSPB8/HSP22/HSP22 Rabbit pAb (STJ24102) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of HeLa cells using HSPB8/HSP22/HSP22 antibody (STJ24102). Blue: DAPI for nuclear staining.

Anti-HSPB8-antibody (aa1-196) (STJ24102)

SKU:
STJ24102

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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-HSPB8 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:100
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Gene Symbol: HSPB8
Gene ID: 26353
Uniprot ID: HSPB8_HUMAN
Immunogen Region: 1-196
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human HSPB8 (NP_055180.1).
Tissue Specificity Predominantly expressed in skeletal muscle and heart.
Function Displays temperature-dependent chaperone activity.
Protein Name Heat Shock Protein Beta-8
Hspb8
Alpha-Crystallin C Chain
E2-Induced Gene 1 Protein
Protein Kinase H11
Small Stress Protein-Like Protein Hsp22
Database Links Reactome: R-HSA-3371571
Cellular Localisation Cytoplasm
Nucleus
Translocates To Nuclear Foci During Heat Shock
Alternative Antibody Names Anti-Heat Shock Protein Beta-8 antibody
Anti-Hspb8 antibody
Anti-Alpha-Crystallin C Chain antibody
Anti-E2-Induced Gene 1 Protein antibody
Anti-Protein Kinase H11 antibody
Anti-Small Stress Protein-Like Protein Hsp22 antibody
Anti-HSPB8 antibody
Anti-CRYAC antibody
Anti-E2IG1 antibody
Anti-HSP22 antibody
Anti-PP1629 antibody

Information sourced from Uniprot.org

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