• Western blot analysis of extracts of various cell lines, using HSP47/SERPINH1 antibody (STJ11103495) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry of paraffin-embedded rat testis using HSP47/SERPINH1 rabbit polyclonal antibody (STJ11103495) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse testis using HSP47/SERPINH1 rabbit polyclonal antibody (STJ11103495) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using HSP47/SERPINH1 rabbit polyclonal antibody (STJ11103495) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using HSP47/SERPINH1 rabbit polyclonal antibody (STJ11103495) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using HSP47/SERPINH1 rabbit polyclonal antibody (STJ11103495) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HSP47/SERPINH1 antibody (350-418) (STJ11103495)

SKU:
STJ11103495

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HSP47/SERPINH1 (350-418) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SERPINH1
Gene ID: 871
Uniprot ID: SERPH_HUMAN
Immunogen Region: 350-418
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-418 of human HSP47/HSP47/SERPINH1 (NP_001193943.1).
Immunogen Sequence: SVFHATAFELDTDGNPFDQD IYGREELRSPKLFYADHPFI FLVRDTQSGSLLFIGRLVRP KGDKMRDEL
Function Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Protein Name Serpin H1
47 Kda Heat Shock Protein
Arsenic-Transactivated Protein 3
Astp3
Cell Proliferation-Inducing Gene 14 Protein
Collagen-Binding Protein
Colligin
Rheumatoid Arthritis-Related Antigen Ra-A47
Database Links Reactome: R-HSA-1650814
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative Antibody Names Anti-Serpin H1 antibody
Anti-47 Kda Heat Shock Protein antibody
Anti-Arsenic-Transactivated Protein 3 antibody
Anti-Astp3 antibody
Anti-Cell Proliferation-Inducing Gene 14 Protein antibody
Anti-Collagen-Binding Protein antibody
Anti-Colligin antibody
Anti-Rheumatoid Arthritis-Related Antigen Ra-A47 antibody
Anti-SERPINH1 antibody
Anti-CBP1 antibody
Anti-CBP2 antibody
Anti-HSP47 antibody
Anti-SERPINH2 antibody
Anti-PIG14 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance