| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-HSD3B1 (1-290) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HSD3B1 |
| Gene ID: |
3283 |
| Uniprot ID: |
3BHS1_HUMAN |
| Immunogen Region: |
1-290 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human HSD3B1 (NP_000853.1). |
| Immunogen Sequence: |
MTGWSCLVTGAGGFLGQRII RLLVKEKELKEIRVLDKAFG PELREEFSKLQNKTKLTVLE GDILDEPFLKRACQDVSVII HTACIIDVFGVTHRESIMNV NVKGTQLLLEACVQASVPVF IYTSSIEVAGPNSYKEIIQN GHEEEPLENTWPAPYPHSKK LAEKAVLAANGWNLKNGGTL YTCALRPMYIYGEGSRFLSA SINEALNNNGILSSVGKFST VNPVYVGNVAWAHILALRA |
| Tissue Specificity | Placenta and skin. Predominantly expressed in mammary gland tissue. |
| Function | A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone. Expected to use NAD(+) as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable). |
| Protein Name | 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 13 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I3-Beta-Hsd I3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase3-Beta-Hydroxysteroid 3-DehydrogenaseDelta-5-3-Ketosteroid IsomeraseDihydrotestosterone OxidoreductaseSteroid Delta-IsomeraseTrophoblast Antigen Fdo161g |
| Database Links | Reactome: R-HSA-193048Reactome: R-HSA-193993Reactome: R-HSA-194002 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinMitochondrion Membrane |
| Alternative Antibody Names | Anti-3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1 antibodyAnti-3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I antibodyAnti-3-Beta-Hsd I antibodyAnti-3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase antibodyAnti-3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase antibodyAnti-3-Beta-Hydroxysteroid 3-Dehydrogenase antibodyAnti-Delta-5-3-Ketosteroid Isomerase antibodyAnti-Dihydrotestosterone Oxidoreductase antibodyAnti-Steroid Delta-Isomerase antibodyAnti-Trophoblast Antigen Fdo161g antibodyAnti-HSD3B1 antibodyAnti-3BH antibodyAnti-HSDB3A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
