• Western blot analysis of lysates from wild type (WT) and HPRT1 knockout (KO) 293T cells, using [KO Validated] HPRT1 Rabbit polyclonal antibody (STJ27659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of various lysates using [KO Validated] HPRT1 Rabbit polyclonal antibody (STJ27659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HPRT1 antibody (1-218) (STJ27659)

SKU:
STJ27659

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HPRT1 (1-218) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HPRT1
Gene ID: 3251
Uniprot ID: HPRT_HUMAN
Immunogen Region: 1-218
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human HPRT1 (NP_000185.1).
Immunogen Sequence: MATRSPGVVISDDEPGYDLD LFCIPNHYAEDLERVFIPHG LIMDRTERLARDVMKEMGGH HIVALCVLKGGYKFFADLLD YIKALNRNSDRSIPMTVDFI RLKSYCNDQSTGDIKVIGGD DLSTLTGKNVLIVEDIIDTG KTMQTLLSLVRQYNPKMVKV ASLLVKRTPRSVGYKPDFVG FEIPDKFVVGYALDYNEYFR DLNHVCVISETGKAKYKA
Function Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Protein Name Hypoxanthine-Guanine Phosphoribosyltransferase
Hgprt
Hgprtase
Database Links Reactome: R-HSA-74217
Reactome: R-HSA-9734281
Reactome: R-HSA-9748787
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Hypoxanthine-Guanine Phosphoribosyltransferase antibody
Anti-Hgprt antibody
Anti-Hgprtase antibody
Anti-HPRT1 antibody
Anti-HPRT antibody

Information sourced from Uniprot.org

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