• Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g HNRNPA0 antibody (STJ27825). Western blot was performed from the immunoprecipitate using HNRNPA0 antibody (STJ27825) at a dilution of 1:1000.
  • Western blot analysis of various lysates using HNRNPA0 Rabbit polyclonal antibody (STJ27825) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HNRNPA0 antibody (1-180) (STJ27825)

SKU:
STJ27825

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNRNPA0
Gene ID: 10949
Uniprot ID: ROA0_HUMAN
Immunogen Region: 1-180
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HNRNPA0 (NP_006796.1).
Immunogen Sequence: MENSQLCKLFIGGLNVQTSE SGLRGHFEAFGTLTDCVVVV NPQTKRSRCFGFVTYSNVEE ADAAMAASPHAVDGNTVELK RAVSREDSARPGAHAKVKKL FVGGLKGDVAEGDLIEHFSQ FGTVEKAEIIADKQSGKKRG FGFVYFQNHDAADKAAVVKF HPIQGHRVEVKKAVPKEDIY
Post Translational Modifications Phosphorylated at Ser-84 by MAPKAPK2 in response to LPS treatment, promoting stabilization of GADD45A mRNA. Arg-291 is dimethylated, probably to asymmetric dimethylarginine.
Function mRNA-binding component of ribonucleosomes. Specifically binds AU-rich element (ARE)-containing mRNAs. Involved in post-transcriptional regulation of cytokines mRNAs.
Protein Name Heterogeneous Nuclear Ribonucleoprotein A0
Hnrnp A0
Cellular Localisation Nucleus
Component Of Ribonucleosomes
Alternative Antibody Names Anti-Heterogeneous Nuclear Ribonucleoprotein A0 antibody
Anti-Hnrnp A0 antibody
Anti-HNRNPA0 antibody
Anti-HNRPA0 antibody

Information sourced from Uniprot.org

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