• Western blot analysis of extracts of various cell lines, using HMOX2 antibody (STJ114586) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HMOX2 antibody (1-270) (STJ114586)

SKU:
STJ114586

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HMOX2 (1-270) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMOX2
Gene ID: 3163
Uniprot ID: HMOX2_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human HMOX2 (NP_002125.3).
Immunogen Sequence: MSAEVETSEGVDESEKKNSG ALEKENQMRMADLSELLKEG TKEAHDRAENTQFVKDFLKG NIKKELFKLATTALYFTYSA LEEEMERNKDHPAFAPLYFP MELHRKEALTKDMEYFFGEN WEEQVQCPKAAQKYVERIHY IGQNEPELLVAHAYTRYMGD LSGGQVLKKVAQRALKLPST GEGTQFYLFENVDNAQQFKQ LYRARMNALDLNMKTKERIV EEANKAFEYNMQIFNELDQ
Post Translational Modifications A soluble form arises by proteolytic removal of the membrane anchor.
Function Heme oxygenase 2: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron. Heme oxygenase 2 soluble form: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.
Protein Name Heme Oxygenase 2
Ho-2 Cleaved Into - Heme Oxygenase 2 Soluble Form
Database Links Reactome: R-HSA-189483
Reactome: R-HSA-6798695
Reactome: R-HSA-8980692
Reactome: R-HSA-917937
Reactome: R-HSA-9707564
Cellular Localisation Microsome Membrane
Single-Pass Type Iv Membrane Protein
Cytoplasmic Side
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Heme Oxygenase 2 antibody
Anti-Ho-2 Cleaved Into - Heme Oxygenase 2 Soluble Form antibody
Anti-HMOX2 antibody
Anti-HO2 antibody

Information sourced from Uniprot.org

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