• Western blot analysis of extracts of various cell lines, using HLX antibody (STJ115277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-HLX antibody (80-280) (STJ115277)

SKU:
STJ115277

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HLX (80-280) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HLX
Gene ID: 3142
Uniprot ID: HLX_HUMAN
Immunogen Region: 80-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-280 of human HLX (NP_068777.1).
Immunogen Sequence: HPHASFQAAARSPLRPTPVV APSEVPAGFPQRLSPLSAAY HHHHPQQQQQQQQPQQQQPP PPPRAGALQPPASGTRVVPN PHHSGSAPAPSSKDLKFGID RILSAEFDPKVKEGNTLRDL TSLLTGGRPAGVHLSGLQPS AGQFFASLDPINEASAILSP LNSNPRNSVQHQFQDTFPGP YAVLTKDTMPQTYKRKRSWS R
Tissue Specificity Low level in normal B and T-cells, high level in activated lymphocytes and monocytes. Also found in thymus, tonsil, bone marrow, developing vessels, and fetal brain.
Function Transcription factor required for TBX21/T-bet-dependent maturation of Th1 cells as well as maintenance of Th1-specific gene expression. Involved in embryogenesis and hematopoiesis.
Protein Name H2.0-Like Homeobox Protein
Homeobox Protein Hb24
Homeobox Protein Hlx1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-H2.0-Like Homeobox Protein antibody
Anti-Homeobox Protein Hb24 antibody
Anti-Homeobox Protein Hlx1 antibody
Anti-HLX antibody
Anti-HLX1 antibody

Information sourced from Uniprot.org

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