• Western blot analysis of extracts of mouse liver, using LAMP1 antibody (STJ113156) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of HeLa cells using LAMP1 antibody (STJ113156) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using LAMP1 antibody (STJ113156) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-LAMP1 antibody (51-270) (STJ113156)

SKU:
STJ113156

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LAMP1 (51-270) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LAMP1
Gene ID: 3916
Uniprot ID: LAMP1_HUMAN
Immunogen Region: 51-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 51-270 of human LAMP1 (NP_005552.3).
Immunogen Sequence: SVNYDTKSGPKNMTFDLPSD ATVVLNRSSCGKENTSDPSL VIAFGRGHTLTLNFTRNATR YSVQLMSFVYNLSDTHLFPN ASSKEIKTVESITDIRADID KKYRCVSGTQVHMNNVTVTL HDATIQAYLSNSSFSRGETR CEQDRPSPTTAPPAPPSPSP SPVPKSPSVDKYNVSGTNGT CLLASMGLQLNLTYERKDNT TVTRLLNINPNKTSASGSCG
Post Translational Modifications O- and N-glycosylated.some of the 18 N-linked glycans are polylactosaminoglycans. (Microbial infection) The glycosylation of Asn-76 is essential for Lassa virus entry into cells.
Function Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis. Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity. Also plays an important role in NK-cells cytotoxicity. Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule. In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content. Presents carbohydrate ligands to selectins. (Microbial infection) Acts as a receptor for Lassa virus glycoprotein. Promotes also fusion of the virus with host membrane in less acidic endosomes. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.
Protein Name Lysosome-Associated Membrane Glycoprotein 1
Lamp-1
Lysosome-Associated Membrane Protein 1
Cd107 Antigen-Like Family Member A
Cd Antigen Cd107a
Database Links Reactome: R-HSA-6798695
Cellular Localisation Lysosome Membrane
Single-Pass Type I Membrane Protein
Endosome Membrane
Late Endosome Membrane
Cell Membrane
Cytolytic Granule Membrane
This Protein Shuttles Between Lysosomes
Endosomes
And The Plasma Membrane
Colocalizes With Osbpl1a At The Late Endosome
Alternative Antibody Names Anti-Lysosome-Associated Membrane Glycoprotein 1 antibody
Anti-Lamp-1 antibody
Anti-Lysosome-Associated Membrane Protein 1 antibody
Anti-Cd107 Antigen-Like Family Member A antibody
Anti-Cd Antigen Cd107a antibody
Anti-LAMP1 antibody

Information sourced from Uniprot.org

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