| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HCK |
| Gene ID: | 3055 |
| Uniprot ID: | HCK_HUMAN |
| Immunogen Region: | 1-220 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human HCK (NP_001165600.1). |
| Immunogen Sequence: | MGCMKSKFLQVGGNTFSKTE TSASPHCPVYVPDPTSTIKP GPNSHNSNTPGIREAGSEDI IVVALYDYEAIHHEDLSFQK GDQMVVLEESGEWWKARSLA TRKEGYIPSNYVARVDSLET EEWFFKGISRKDAERQLLAP GNMLGSFMIRDSETTKGSYS LSVRDYDPRQGDTVKHYKIR TLDNGGFYISPRSTFSTLQE LVDHYKKGNDGLCQKLSVPC |
| Tissue Specificity | Detected in monocytes and neutrophils (at protein level). Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes. Detected in tonsil. |
| Post Translational Modifications | Phosphorylated on several tyrosine residues. Autophosphorylated. Becomes rapidly phosphorylated upon activation of the immunoglobulin receptors FCGR1A and FCGR2A. Phosphorylation by the BCR-ABL fusion protein mediates activation of HCK. Phosphorylation at Tyr-411 increases kinase activity. Phosphorylation at Tyr-522 inhibits kinase activity. Kinase activity is not required for phosphorylation at Tyr-522, suggesting that this site is a target of other kinases. Ubiquitinated by CBL, leading to its degradation via the proteasome. Isoform 2 palmitoylation at position 2 requires prior myristoylation. Palmitoylation at position 3 is required for caveolar localization of isoform 2. |
| Function | Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS. |
| Protein Name | Tyrosine-Protein Kinase Hck Hematopoietic Cell Kinase Hemopoietic Cell Kinase P59-Hck/P60-Hck P59hck P61hck |
| Database Links | Reactome: R-HSA-164944 Reactome: R-HSA-2029481 Reactome: R-HSA-912631 Reactome: R-HSA-9664323 Reactome: R-HSA-9664422 Reactome: R-HSA-9674555 Reactome: R-HSA-9680350 Reactome: R-HSA-9705462 Reactome: R-HSA-9706374 |
| Cellular Localisation | Isoform 1: Lysosome Membrane Lipid-Anchor Cell Projection Podosome Membrane Cytoplasm Cytosol Associated With Specialized Secretory Lysosomes Called Azurophil Granules At Least Half Of This Isoform Is Found In The Cytoplasm Some Of This Fraction Is Myristoylated Isoform 2: Cell Membrane Caveola Cell Junction Focal Adhesion Cytoskeleton Golgi Apparatus Cytoplasmic Vesicle Lysosome Nucleus 20% Of This Isoform Is Associated With Caveolae Localization At The Cell Membrane And At Caveolae Requires Palmitoylation At Cys-3 Colocalizes With The Actin Cytoskeleton At Focal Adhesions Secretory Vesicle |
| Alternative Antibody Names | Anti-Tyrosine-Protein Kinase Hck antibody Anti-Hematopoietic Cell Kinase antibody Anti-Hemopoietic Cell Kinase antibody Anti-P59-Hck/P60-Hck antibody Anti-P59hck antibody Anti-P61hck antibody Anti-HCK antibody |
Information sourced from Uniprot.org