• Western blot analysis of extracts of various cell lines, using GTF2H2 Antibody (STJ27270) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GTF2H2 antibody (1-100) (STJ27270)

SKU:
STJ27270

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GTF2H2 (1-100) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GTF2H2
Gene ID: 2966
Uniprot ID: TF2H2_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GTF2H2 (NP_001506.1).
Immunogen Sequence: MDEEPERTKRWEGGYERTWE ILKEDESGSLKATIEDILFK AKRKRVFEHHGQVRLGMMRH LYVVVDGSRTMEDQDLKPNR LTCTLKLLEYFVEEYFDQNP
Tissue Specificity Widely expressed, with higher expression in skeletal muscle.
Function Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. The N-terminus of GTF2H2 interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter.
Protein Name General Transcription Factor Iih Subunit 2
Basic Transcription Factor 2 44 Kda Subunit
Btf2 P44
General Transcription Factor Iih Polypeptide 2
Tfiih Basal Transcription Factor Complex P44 Subunit
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-113418
Reactome: R-HSA-167152
Reactome: R-HSA-167158
Reactome: R-HSA-167160
Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-167200
Reactome: R-HSA-167246
Reactome: R-HSA-427413
Reactome: R-HSA-5696395
Reactome: R-HSA-5696400
Reactome: R-HSA-674695
Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6796648
Reactome: R-HSA-72086
Reactome: R-HSA-73762
Reactome: R-HSA-73772
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-73863
Reactome: R-HSA-75953
Reactome: R-HSA-75955
Reactome: R-HSA-76042
Reactome: R-HSA-77075
Cellular Localisation Nucleus
Alternative Antibody Names Anti-General Transcription Factor Iih Subunit 2 antibody
Anti-Basic Transcription Factor 2 44 Kda Subunit antibody
Anti-Btf2 P44 antibody
Anti-General Transcription Factor Iih Polypeptide 2 antibody
Anti-Tfiih Basal Transcription Factor Complex P44 Subunit antibody
Anti-GTF2H2 antibody
Anti-BTF2P44 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance