• Immunofluorescence analysis of A549 cells using GTF2F2 antibody (STJ115458).
  • Immunohistochemistry analysis of paraffin-embedded human kidney cancer using GTF2F2 antibody (STJ115458) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using GTF2F2 antibody (STJ115458) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using GTF2F2 antibody (STJ115458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-GTF2F2 antibody (1-249) (STJ115458)

SKU:
STJ115458

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GTF2F2 (1-249) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GTF2F2
Gene ID: 2963
Uniprot ID: T2FB_HUMAN
Immunogen Region: 1-249
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human GTF2F2 (NP_004119.1).
Immunogen Sequence: MAERGELDLTGAKQNTGVWL VKVPKYLSQQWAKASGRGEV GKLRIAKTQGRTEVSFTLNE DLANIHDIGGKPASVSAPRE HPFVLQSVGGQTLTVFTESS SDKLSLEGIVVQRAECRPAA SENYMRLKRLQIEESSKPVR LSQQLDKVVTTNYKPVANHQ YNIEYERKKKEDGKRARADK QHVLDMLFSAFEKHQYYNLK DLVDITKQPVVYLKEILKEI GVQNVKGIHKNTWELKPEY
Function TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB.
Protein Name General Transcription Factor Iif Subunit 2
General Transcription Factor Iif 30 Kda Subunit
Transcription Initiation Factor Iif Subunit Beta
Tfiif-Beta
Transcription Initiation Factor Rap30
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-113418
Reactome: R-HSA-167152
Reactome: R-HSA-167158
Reactome: R-HSA-167160
Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-167200
Reactome: R-HSA-167238
Reactome: R-HSA-167242
Reactome: R-HSA-167243
Reactome: R-HSA-167246
Reactome: R-HSA-167287
Reactome: R-HSA-167290
Reactome: R-HSA-168325
Reactome: R-HSA-674695
Reactome: R-HSA-6796648
Reactome: R-HSA-6803529
Reactome: R-HSA-6807505
Reactome: R-HSA-72086
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Reactome: R-HSA-72203
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-75953
Reactome: R-HSA-75955
Reactome: R-HSA-76042
Reactome: R-HSA-77075
Reactome: R-HSA-8851708
Reactome: R-HSA-9018519
Cellular Localisation Nucleus
Alternative Antibody Names Anti-General Transcription Factor Iif Subunit 2 antibody
Anti-General Transcription Factor Iif 30 Kda Subunit antibody
Anti-Transcription Initiation Factor Iif Subunit Beta antibody
Anti-Tfiif-Beta antibody
Anti-Transcription Initiation Factor Rap30 antibody
Anti-GTF2F2 antibody
Anti-RAP30 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance