• Western blot analysis of extracts of various cell lines, using GST3/GSTP1 antibody (STJ11101599) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using GST3/GSTP1 rabbit monoclonal antibody (STJ11101599) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using GST3/GSTP1 rabbit monoclonal antibody (STJ11101599) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma using GST3/GSTP1 rabbit monoclonal antibody (STJ11101599) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using GST3/GSTP1 rabbit monoclonal antibody (STJ11101599) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using GST3/GSTP1 rabbit monoclonal antibody (STJ11101599) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using GST3/GSTP1 rabbit monoclonal antibody (STJ11101599) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using GST3/GSTP1 rabbit monoclonal antibody (STJ11101599) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using GST3/GSTP1 rabbit monoclonal antibody (STJ11101599) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-GSTP1 antibody (100-201) [S9MR] (STJ11101599)

SKU:
STJ11101599

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GST3/GSTP1 (100-201) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GSTP1
Gene ID: 2950
Uniprot ID: GSTP1_HUMAN
Immunogen Region: 100-201
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-201 of human GST3/GSTP1 (P09211).
Immunogen Sequence: LRCKYISLIYTNYEAGKDDY VKALPGQLKPFETLLSQNQG GKTFIVGDQISFADYNLLDL LLIHEVLAPGCLDAFPLLSA YVGRLSARPKLKAFLASPEY VN
Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). Participates in the formation of novel hepoxilin regioisomers. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.
Protein Name Glutathione S-Transferase P
Gst Class-Pi
Gstp1-1
Database Links Reactome: R-HSA-156590
Reactome: R-HSA-3299685
Reactome: R-HSA-6798695
Reactome: R-HSA-9753281
Cellular Localisation Cytoplasm
Mitochondrion
Nucleus
The 83 N-Terminal Amino Acids Function As Un Uncleaved Transit Peptide
And Arginine Residues Within It Are Crucial For Mitochondrial Localization
Alternative Antibody Names Anti-Glutathione S-Transferase P antibody
Anti-Gst Class-Pi antibody
Anti-Gstp1-1 antibody
Anti-GSTP1 antibody
Anti-FAEES3 antibody
Anti-GST3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance