• Western blot analysis of extracts of various cell lines, using GM2A antibody (STJ28688) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5min.

Anti-GM2A antibody (24-193) (STJ28688)

SKU:
STJ28688

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GM2A (24-193) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GM2A
Gene ID: 2760
Uniprot ID: SAP3_HUMAN
Immunogen Region: 24-193
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-193 of human GM2A (NP_000396.2).
Immunogen Sequence: HLKKPSQLSSFSWDNCDEGK DPAVIRSLTLEPDPIIVPGN VTLSVMGSTSVPLSSPLKVD LVLEKEVAGLWIKIPCTDYI GSCTFEHFCDVLDMLIPTGE PCPEPLRTYGLPCHCPFKEG TYSLPKSEFVVPDLELPSWL TTGNYRIESVLSSSGKRLGC IKIAASLKGI
Post Translational Modifications The serines in positions 32 and 33 are absent in 80% of the sequenced protein.
Function The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity. Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. Has cholesterol transfer activity.
Protein Name Ganglioside Gm2 Activator
Cerebroside Sulfate Activator Protein
Gm2-Ap
Sphingolipid Activator Protein 3
Sap-3 Cleaved Into - Ganglioside Gm2 Activator Isoform Short
Database Links Reactome: R-HSA-1660662
Reactome: R-HSA-6798695
Cellular Localisation Lysosome
Alternative Antibody Names Anti-Ganglioside Gm2 Activator antibody
Anti-Cerebroside Sulfate Activator Protein antibody
Anti-Gm2-Ap antibody
Anti-Sphingolipid Activator Protein 3 antibody
Anti-Sap-3 Cleaved Into - Ganglioside Gm2 Activator Isoform Short antibody
Anti-GM2A antibody

Information sourced from Uniprot.org

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