• Western blot analysis of extracts of various cell lines, using GLB1 antibody (STJ115624) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-GLB1 antibody (1-349) (STJ115624)

SKU:
STJ115624

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GLB1 (1-349) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GLB1
Gene ID: 2720
Uniprot ID: BGAL_HUMAN
Immunogen Region: 1-349
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human GLB1 (NP_000395.3).
Immunogen Sequence: TSYDYDAPLSEAGDLTEKYF ALRNIIQKFEKVPEGPIPPS TPKFAYGKVTLEKLKTVGAA LDILCPSGPIKSLYPLTFIQ VKQHYGFVLYRTTLPQDCSN PAPLSSPLNGVHDRAYVAVD GIPQGVLERNNVITLNITGK AGATLDLLVENMGRVNYGAY INDFKGLVSNLTLSSNILTD WTIFPLDTEDAVCSHLGGWG HRDSGHHDEAWAHNSSNYTL PAFYMGNFSIPSGIPDLPQ
Tissue Specificity Detected in placenta (at protein level). Detected in fibroblasts and testis.
Function Isoform 1: Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Isoform 2: Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.
Protein Name Beta-Galactosidase
Acid Beta-Galactosidase
Lactase
Elastin Receptor 1
Database Links Reactome: R-HSA-1660662
Reactome: R-HSA-2022857
Reactome: R-HSA-2024096
Reactome: R-HSA-2206308
Reactome: R-HSA-4085001
Reactome: R-HSA-4341670
Reactome: R-HSA-6798695
Cellular Localisation Isoform 1: Lysosome
Isoform 2: Cytoplasm
Perinuclear Region
Localized To The Perinuclear Area Of The Cytoplasm But Not To Lysosomes
Alternative Antibody Names Anti-Beta-Galactosidase antibody
Anti-Acid Beta-Galactosidase antibody
Anti-Lactase antibody
Anti-Elastin Receptor 1 antibody
Anti-GLB1 antibody
Anti-ELNR1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance