• Western blot analysis of various lysates using F8A1 Rabbit polyclonal antibody (STJ117006) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-F8A1 antibody (200-300) (STJ117006)

SKU:
STJ117006

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-F8A1 (200-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: F8A1.F8A2.F8A3
Gene ID: 474383/474384/8263
Uniprot ID: HAP40_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human F8A1 (NP_036283.2).
Immunogen Sequence: ALAVFTRMQRLAREHGSHPV QSLPPPPPPAPQPGPGATPA LPAALLPPNSGSAAPSPAAL GAFSDVLVRCEVSRVLLLLL LQPPPAKLLPEHAQTLEKYS W
Tissue Specificity Produced abundantly in a wide variety of cell types.
Function RAB5A effector molecule that is involved in vesicular trafficking of early endosomes. Mediates the recruitment of HTT by RAB5A onto early endosomes. The HTT-F8A1/F8A2/F8A3-RAB5A complex stimulates early endosomal interaction with actin filaments and inhibits interaction with microtubules, leading to the reduction of endosome motility.
Protein Name 40-Kda Huntingtin-Associated Protein
Hap40
Cpg Island Protein
Factor Viii Intron 22 Protein
Cellular Localisation Cytoplasm
Nucleus
Early Endosome
Nuclear Body
Diffuse Presence In The Cytoplasm And Accumulation In The Nucleus
In Absence Of Htt
F8a1/F8a2/F8a3 Is Concentred In Cytoplasm
Colocalized With Htt In Endosomes
In Neuron Found In Intranuclear Structures
The Intranuclear Rodlets (Inrs)
Also Known As Rodlets Of Roncoroni
In Association With Ubiquitin
Alternative Antibody Names Anti-40-Kda Huntingtin-Associated Protein antibody
Anti-Hap40 antibody
Anti-Cpg Island Protein antibody
Anti-Factor Viii Intron 22 Protein antibody
Anti-F8A1.F8A2.F8A3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance