| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EXO1 (601-846) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EXO1 |
| Gene ID: |
9156 |
| Uniprot ID: |
EXO1_HUMAN |
| Immunogen Region: |
601-846 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 601-846 of human EXO1 (NP_006018.4). |
| Immunogen Sequence: |
TLGTLRSCFSWSGGLGDFSR TPSPSPSTALQQFRRKSDSP TSLPENNMSDVSQLKSEESS DDESHPLREEACSSQSQESG EFSLQSSNASKLSQCSSKDS DSEESDCNIKLLDSQSDQTS KLRLSHFSKKDTPLRNKVPG LYKSSSADSLSTTKIKPLGP ARASGLSKKPASIQKRKHHN AENKPGLQIKLNELWKNFGF KKDSEKLPPCKKPLSPVRDN IQLTPEAEEDIFNKPECGR |
| Tissue Specificity | Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. |
| Post Translational Modifications | Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilization/degradation of the protein. |
| Function | 5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis. |
| Protein Name | Exonuclease 1Hexo1Exonuclease IHexoi |
| Database Links | Reactome: R-HSA-5358565Reactome: R-HSA-5358606Reactome: R-HSA-5685938Reactome: R-HSA-5685942Reactome: R-HSA-5693554Reactome: R-HSA-5693568Reactome: R-HSA-5693579Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709570Reactome: R-HSA-9709603 |
| Cellular Localisation | NucleusColocalizes With Pcna To Discrete Nuclear Foci In S-Phase |
| Alternative Antibody Names | Anti-Exonuclease 1 antibodyAnti-Hexo1 antibodyAnti-Exonuclease I antibodyAnti-Hexoi antibodyAnti-EXO1 antibodyAnti-EXOI antibodyAnti-HEX1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
