| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ERO1LB (34-230) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ERO1B |
| Gene ID: |
56605 |
| Uniprot ID: |
ERO1B_HUMAN |
| Immunogen Region: |
34-230 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 34-230 of human ERO1LB (NP_063944.3). |
| Immunogen Sequence: |
QVTGVLDDCLCDIDSIDNFN TYKIFPKIKKLQERDYFRYY KVNLKRPCPFWAEDGHCSIK DCHVEPCPESKIPVGIKAGH SNKYLKMANNTKELEDCEQA NKLGAINSTLSNQSKEAFID WARYDDSRDHFCELDDERSP AAQYVDLLLNPERYTGYKGT SAWRVWNSIYEENCFKPRSV YRPLNPLAPSRGEDDGE |
| Tissue Specificity | Highly expressed in the digestive tract, including the duodenum and lower digestive tract. In the stomach, highly expressed in enzyme-producing chief cells (at protein level). In the pancreas, expressed in islets of Langerhans and, at lower levels, in enzyme-secreting cells (at protein level). Detected at low level in many other tissues. |
| Post Translational Modifications | N-glycosylated. The Cys-90/Cys-95 and Cys-393/Cys-396 disulfide bonds constitute the redox-active center. The Cys-90/Cys-95 disulfide bond accepts electron from P4HB and funnel them to the active site disulfide Cys-393/Cys-396. The Cys-81/Cys-390 disulfide bond may be critical for structural stability. Two long-range disulfide bonds participate in loose feedback regulation. The Cys-90/Cys-130 disulfide bond may be the predominant regulatory switch to modulate the catalytic activity, while the Cys-100/Cys-262 disulfide bond may play an auxiliary regulatory role. |
| Function | Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Other protein disulfide isomerase family members can also be reoxidized, but at lower rates compared to P4HB, including PDIA2 (50% of P4HB reoxidation rate), as well as PDIA3, PDIA4, PDIA6 and NXNDC12 (<10%). Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. May be involved in oxidative proinsulin folding in pancreatic cells, hence may play a role in glucose homeostasis. |
| Protein Name | Ero1-Like Protein BetaEro1-L-BetaEndoplasmic Reticulum Oxidoreductase BetaEndoplasmic Reticulum Oxidoreductin-1-Like Protein BOxidoreductin-1-L-Beta |
| Database Links | Reactome: R-HSA-264876 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinLumenal SideThe Association With Erp44 May Be Essential For Its Retention In The Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Ero1-Like Protein Beta antibodyAnti-Ero1-L-Beta antibodyAnti-Endoplasmic Reticulum Oxidoreductase Beta antibodyAnti-Endoplasmic Reticulum Oxidoreductin-1-Like Protein B antibodyAnti-Oxidoreductin-1-L-Beta antibodyAnti-ERO1B antibodyAnti-ERO1LB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and Prion Protein knockout (KO) HeLa cells, using [KO Validated] Prion Protein Rabbit polyclonal antibody (STJ11100031) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89103/357560/STJ11100031_1__14868.1713121685.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Prion Protein knockout (KO) HeLa cells, using [KO Validated] Prion Protein Rabbit polyclonal antibody (STJ11100031) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
