• Western blot analysis of extracts of various cell lines, using eIF4A1 rabbit monoclonal antibody (STJ11101363) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of NIH-3T3 cells using eIF4A1 rabbit monoclonal antibody (STJ11101363) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug eIF4A1 antibody (STJ11101363). Western blot was performed from the immunoprecipitate using eIF4A1 (STJ11101363) at a dilition of 1:1000.

Anti-EIF4A1 antibody (1-100) [S3MR] (STJ11101363)

SKU:
STJ11101363

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-eIF4A1 (1-100) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF4A1
Gene ID: 1973
Uniprot ID: IF4A1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4A1 (P60842).
Immunogen Sequence: MSASQDSRSRDNGPDGMEPE GVIESNWNEIVDSFDDMNLS ESLLRGIYAYGFEKPSAIQQ RAILPCIKGYDVIAQAQSGT GKTATFAISILQQIELDLKA
Function ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Protein Name Eukaryotic Initiation Factor 4a-I
Eif-4a-I
Eif4a-I
Atp-Dependent Rna Helicase Eif4a-1
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-156827
Reactome: R-HSA-429947
Reactome: R-HSA-72649
Reactome: R-HSA-72662
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Alternative Antibody Names Anti-Eukaryotic Initiation Factor 4a-I antibody
Anti-Eif-4a-I antibody
Anti-Eif4a-I antibody
Anti-Atp-Dependent Rna Helicase Eif4a-1 antibody
Anti-EIF4A1 antibody
Anti-DDX2A antibody
Anti-EIF4A antibody

Information sourced from Uniprot.org

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