• Western blot analysis of extracts of various cell lines, using EHBP1 antibody (STJ111640) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-EHBP1 antibody (1-100) (STJ111640)

SKU:
STJ111640

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EHBP1 (1-100) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EHBP1
Gene ID: 23301
Uniprot ID: EHBP1_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human EHBP1 (NP_056067.2).
Immunogen Sequence: MASVWKRLQRVGKHASKFQF VASYQELMVECTKKWQPDKL VVVWTRRSRRKSSKAHSWQP GIKNPYRGVVVWPVPENIEI TVTLFKDPHAEEFEDKEWTF
Post Translational Modifications Prenylated (Probable). Farnelysation (predominant) and geranylgeranylation has been observed in vitro.
Function May play a role in actin reorganization. Links clathrin-mediated endocytosis to the actin cytoskeleton. May act as Rab effector protein and play a role in vesicle trafficking. Required for perinuclear sorting and insulin-regulated recycling of SLC2A4/GLUT4 in adipocytes.
Protein Name Eh Domain-Binding Protein 1
Cellular Localisation Cytoplasm
Membrane
Endosome
Mostly Found In Cytosol And Plasma Membrane
Alternative Antibody Names Anti-Eh Domain-Binding Protein 1 antibody
Anti-EHBP1 antibody
Anti-KIAA0903 antibody
Anti-NACSIN antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance