• Western blot analysis of extracts of various cell lines, using EDAR antibody (STJ116040) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-EDAR antibody (27-187) (STJ116040)

SKU:
STJ116040

$228.83 - $606.83
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EDAR (27-187) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EDAR
Gene ID: 10913
Uniprot ID: EDAR_HUMAN
Immunogen Region: 27-187
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-187 of human EDAR (NP_071731.1).
Immunogen Sequence: EYSNCGENEYYNQTTGLCQE CPPCGPGEEPYLSCGYGTKD EDYGCVPCPAEKFSKGGYQI CRRHKDCEGFFRATVLTPGD MENDAECGPCLPGYYMLENR PRNIYGMVCYSCLLAPPNTK ECVGATSGASANFPGTSGSS TLSPFQHAHKELSGQGHLAT A
Tissue Specificity Detected in fetal kidney, lung, skin and cultured neonatal epidermal keratinocytes. Not detected in lymphoblast and fibroblast cell lines.
Function Receptor for EDA isoform A1, but not for EDA isoform A2. Mediates the activation of NF-kappa-B and JNK. May promote caspase-independent cell death.
Protein Name Tumor Necrosis Factor Receptor Superfamily Member Edar
Anhidrotic Ectodysplasin Receptor 1
Downless Homolog
Eda-A1 Receptor
Ectodermal Dysplasia Receptor
Ectodysplasin-A Receptor
Database Links Reactome: R-HSA-5669034
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Tumor Necrosis Factor Receptor Superfamily Member Edar antibody
Anti-Anhidrotic Ectodysplasin Receptor 1 antibody
Anti-Downless Homolog antibody
Anti-Eda-A1 Receptor antibody
Anti-Ectodermal Dysplasia Receptor antibody
Anti-Ectodysplasin-A Receptor antibody
Anti-EDAR antibody
Anti-DL antibody

Information sourced from Uniprot.org

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