• Immunofluorescence analysis of A-549 cells using EAF2 antibody (STJ29199).
  • Western blot analysis of extracts of various cell lines, using EAF2 antibody (STJ29199) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-EAF2 antibody (1-170) (STJ29199)

SKU:
STJ29199

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EAF2 (1-170) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EAF2
Gene ID: 55840
Uniprot ID: EAF2_HUMAN
Immunogen Region: 1-170
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human EAF2 (NP_060926.2).
Immunogen Sequence: MNSAAGFSHLDRRERVLKLG ESFEKQPRCAFHTVRYDFKP ASIDTSSEGYLEVGEGEQVT ITLPNIEGSTPPVTVFKGSK KPYLKECILIINHDTGECRL EKLSSNITVKKTRVEGSSKI QYRKEQQQQQMWNSARTPNL VKHSPSEDKMSPASPIDDIE RELKAEASLM
Tissue Specificity Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymph node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes.
Function Acts as a transcriptional transactivator of TCEA1 elongation activity. Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.
Protein Name Ell-Associated Factor 2
Testosterone-Regulated Apoptosis Inducer And Tumor Suppressor Protein
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-674695
Reactome: R-HSA-75955
Cellular Localisation Nucleus Speckle
Alternative Antibody Names Anti-Ell-Associated Factor 2 antibody
Anti-Testosterone-Regulated Apoptosis Inducer And Tumor Suppressor Protein antibody
Anti-EAF2 antibody
Anti-TRAITS antibody
Anti-BM-040 antibody

Information sourced from Uniprot.org

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