• Immunofluorescence analysis of MCF7 cells using DR1 antibody (STJ115262).
  • Immunohistochemistry analysis of paraffin-embedded human gastric cancer using DR1 antibody (STJ115262) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of mouse spleen, using DR1 antibody (STJ115262) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DR1 antibody (1-176) (STJ115262)

SKU:
STJ115262

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DR1 (1-176) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DR1
Gene ID: 1810
Uniprot ID: NC2B_HUMAN
Immunogen Region: 1-176
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-176 of human DR1 (NP_001929.1).
Immunogen Sequence: MASSSGNDDDLTIPRAAINK MIKETLPNVRVANDARELVV NCCTEFIHLISSEANEICNK SEKKTISPEHVIQALESLGF GSYISEVKEVLQECKTVALK RRKASSRLENLGIPEEELLR QQQELFAKARQQQAELAQQE WLQMQQAAQQAQLAAASASA SNQAGSSQDEEDDDDI
Post Translational Modifications Phosphorylation regulates its interaction with TBP. Not phosphorylated when bound to DRAP1.
Function The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.
Protein Name Protein Dr1
Down-Regulator Of Transcription 1
Negative Cofactor 2-Beta
Nc2-Beta
Tata-Binding Protein-Associated Phosphoprotein
Database Links Reactome: R-HSA-3214847
Reactome: R-HSA-9772755
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Protein Dr1 antibody
Anti-Down-Regulator Of Transcription 1 antibody
Anti-Negative Cofactor 2-Beta antibody
Anti-Nc2-Beta antibody
Anti-Tata-Binding Protein-Associated Phosphoprotein antibody
Anti-DR1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance