• Western blot analysis of lysates from Rat lung, using DDX28 Rabbit polyclonal antibody (STJ119770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-DDX28 antibody (371-540) (STJ119770)

SKU:
STJ119770

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DDX28 (371-540) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDX28
Gene ID: 55794
Uniprot ID: DDX28_HUMAN
Immunogen Region: 371-540
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 371-540 of human DDX28 (NP_060850.2).
Immunogen Sequence: RLKGADKVAELVHILKHRDR AERTGPSGTVLVFCNSSSTV NWLGYILDDHKIQHLRLQGQ MPALMRVGIFQSFQKSSRDI LLCTDIASRGLDSTGVELVV NYDFPPTLQDYIHRAGRVGR VGSEVPGTVISFVTHPWDVS LVQKIELAARRRRSLPGLAS SVKEPLPQAT
Tissue Specificity Expressed in all tissues tested, including brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, leukocytes, colon, small intestine, ovary and prostate.
Function Plays an essential role in facilitating the proper assembly of the mitochondrial large ribosomal subunit and its helicase activity is essential for this function. May be involved in RNA processing or transport. Has RNA and Mg(2+)-dependent ATPase activity.
Protein Name Probable Atp-Dependent Rna Helicase Ddx28
Mitochondrial Dead Box Protein 28
Cellular Localisation Nucleus
Mitochondrion
Mitochondrion Matrix
Mitochondrion Nucleoid
Transported Between These Two Compartments
Nuclear Localization Depends On Active Rna Polymerase Ii Transcription
Localizes To Mitochondrial Rna Granules Found In Close Proximity To The Mitochondrial Nucleoids
Alternative Antibody Names Anti-Probable Atp-Dependent Rna Helicase Ddx28 antibody
Anti-Mitochondrial Dead Box Protein 28 antibody
Anti-DDX28 antibody
Anti-MDDX28 antibody

Information sourced from Uniprot.org

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