| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DDX25 |
| Gene ID: | 29118 |
| Uniprot ID: | DDX25_HUMAN |
| Immunogen Region: | 284-483 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 284-483 of human DDX25 (NP_037396.3). |
| Immunogen Sequence: | DSVWHFAERIIPDPNVIKLR KEELTLNNIRQYYVLCEHRK DKYQALCNIYGSITIGQAII FCQTRRNAKWLTVEMIQDGH QVSLLSGELTVEQRASIIQR FRDGKEKVLITTNVCARGID VKQVTIVVNFDLPVKQGEEP DYETYLHRIGRTGRFGKKGL AFNMIEVDELPSLMKIQDHF NSSIKQLNAEDMDEIEKIDY |
| Tissue Specificity | Highly expressed in the Leydig and germ cells of the testis and weakly expressed in the pituitary and hypothalamus. |
| Post Translational Modifications | Phosphorylated on threonine residues. The phosphorylated form is found in the cytoplasm but not in the nucleus. |
| Function | ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development. |
| Protein Name | Atp-Dependent Rna Helicase Ddx25Dead Box Protein 25Gonadotropin-Regulated Testicular Rna Helicase |
| Cellular Localisation | CytoplasmNucleusDetected In Both Cytoplasm And Nucleus Of Testicular CellsAlso Detected In Chromatoid Bodies Of Round Spermatids |
| Alternative Antibody Names | Anti-Atp-Dependent Rna Helicase Ddx25 antibodyAnti-Dead Box Protein 25 antibodyAnti-Gonadotropin-Regulated Testicular Rna Helicase antibodyAnti-DDX25 antibodyAnti-GRTH antibody |
Information sourced from Uniprot.org