• Western blot analysis of extracts of various cell lines, using DAAM1 antibody (STJ114795) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DAAM1 antibody (769-1068) (STJ114795)

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STJ114795

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DAAM1 (769-1068) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DAAM1
Gene ID: 23002
Uniprot ID: DAAM1_HUMAN
Immunogen Region: 769-1068
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 769-1068 of human DAAM1 (NP_001257449.1).
Immunogen Sequence: FKKKFAERVAEVKPKVEAIR SGSEEVFRSGALKQLLEVVL AFGNYMNKGQRGNAYGFKIS SLNKIADTKSSIDKNITLLH YLITIVENKYPSVLNLNEEL RDIPQAAKVNMTELDKEIST LRSGLKAVETELEYQKSQPP QPGDKFVSVVSQFITVASFS FSDVEDLLAEAKDLFTKAVK HFGEEAGKIQPDEFFGIFDQ FLQAVSEAKQENENMRKKKE EEERRARMEAQLKEQRERE
Tissue Specificity Expressed in all tissues examined.
Function Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells. Together with DAAM2, required for myocardial maturation and sarcomere assembly.
Protein Name Disheveled-Associated Activator Of Morphogenesis 1
Database Links Reactome: R-HSA-4086400
Reactome: R-HSA-5663220
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Cellular Localisation Cytoplasm
Cytoskeleton
Cilium Basal Body
Perinuclear
Alternative Antibody Names Anti-Disheveled-Associated Activator Of Morphogenesis 1 antibody
Anti-DAAM1 antibody
Anti-KIAA0666 antibody

Information sourced from Uniprot.org

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